Short Chain Fatty Acid Metabolism and Energy Metabolism

Maastricht University Medical Centre (MUMC)

Status: Completed

Conditions: Type 2 Diabetes Mellitus; Obesity

Treatments: Other: acetate or placebo infusion

Study type: Interventional

Funder types: Other

Identifiers

NCT01826162

MEC 11-3-079

Details and patient eligibility

About

Gut microbiota is being increasingly recognized as an important factor in fat distribution, insulin sensitivity and glucose and lipid metabolism. Accordingly, the intestinal microbiota could play an important role in the development of obesity and type 2 diabetes mellitus. The role of gut-derived short-chain fatty acids (SCFA), the formation of which is enhanced by microbial fermentation of fibre, is still controversial. At the present time, our understanding of the effects of SCFA on human metabolism (in gut or systemically) is still limited. The investigators hypothesize that the differential availability of SCFA impacts human metabolism differently.

In this placebo controlled, double-blind, randomized crossover pilot study the investigators will validate in overweight/obese healthy male volunteers whether rectal administration of SCFA is a good model for studying the acute metabolic effects of SCFA. For this, it will be investigated if site of administration (in distal or proximal colon) of SCFA differentially affects parameters of substrate and energy metabolism and to test the duration of short-term effects of SCFA administration.

Enrollment: 10 patients

Sex: Male

Ages: 20 to 50 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Overweight, obese men

Exclusion criteria

• athletes
• diabetes mellitus

Trial design

10 participants in 2 patient groups

sigmoidoscopy

Experimental group

Description:

2 actetate concentrations and 1 placebo are administered in randomized order after clipping a catheter in the proximal colon (sigmoidoscopy)

Treatment:

Other: acetate or placebo infusion

colonoscopy

Experimental group

Description:

2 actetate concentrations and 1 placebo are administered in randomized order after clipping a catheter in the distal colon (colonoscopy)

Treatment:

Other: acetate or placebo infusion