Side of Embryo Biopsy Interfering Implantation Potential

Ahmad Mustafa Mohamed Metwalley

Status: Completed

Conditions: Preimplantation Diagnosis

Treatments: Procedure: Vegetal Pole blastomer embryo; Procedure: Animal Pole blastomer embryo

Study type: Interventional

Funder types: Other

Identifiers

NCT02902614

Embryo Biopsy

Details and patient eligibility

About

Selection of side of embryo biopsy that will not interfering with implantation power of developed blastocyst embryo during biopsy procedure arranged for PGT.

Full description

During process of embryo biopsy, it was indicated to avoid contacting cells closer to inner cell mass as it can decrease the quality of embryo and its structural composition. However, recommendation for collection from the trophectoderm (TE) side is indicated.

Absence of differentiation during cleavage-stage or morula stage at day 3 and day 4 respectively, will present the needful to focus a specific location for embryo biopsy during pre-blastocyst stages for embryo going to do genetic testing before implantation or embryo transfer.

In articulation around the second polar body or the animal pole of embryo, collection of the cell from the vegetal pole in crospondence to avoide collection form the animal pole is to be compare the results of implantation for biopsy done using the animal pole side or the random non organized biosy procedure.

Enrollment: 100 patients

Sex: All

Volunteers: No Healthy Volunteers

Inclusion criteria

• grade 1 or 2 embryos at eight cell stage or 8-1/ 8-2 embryo quality.

Exclusion criteria

• grade 3 embryo at day 3, or 8-3 embryos.

Trial design

Primary purpose: Supportive Care

Allocation: Non-Randomized

Interventional model: Crossover Assignment

Masking: None (Open label)

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100 participants in 2 patient groups

Vegetal Pole blastomer embryo

Experimental group

Description:

for day 3 embryo biopsy / cleavage stage: Blastomere collection from opposite side to the 2nd polar body or from vegetal pole.

Treatment:

Procedure: Animal Pole blastomer embryo

Procedure: Vegetal Pole blastomer embryo

Animal Pole blastomer embryo

Experimental group

Description:

for day 3 embryo biopsy / cleavage stage: Blastomere collection from and side around the 2nd polar body and not the vegetal pole.

Treatment:

Procedure: Animal Pole blastomer embryo

Procedure: Vegetal Pole blastomer embryo