Significance of Biological Markers in Patients With Acute Lung Injury/Acute Respiratory Disease

University of Michigan

Status

Completed

Conditions

Respiratory Distress Syndrome, Adult

Treatments

Procedure: Bronchoscopy

Study type

Interventional

Funder types

Other

NIH

Identifiers

NCT00217880

292

P50HL074024 (U.S. NIH Grant/Contract)

Details and patient eligibility

About

The purpose of this study is to identify biological markers of disease in patients with acute lung injury (ALI) that are predictive of either disease susceptibility or prognosis, or that identify novel targets of therapeutic intervention.

Full description

BACKGROUND:

Respiratory failure due to ALI and acute respiratory distress syndrome (ARDS) remains a major health problem despite significant progress in intensive care unit (ICU) care and ventilator management. It is also characterized by an unacceptably high mortality rate despite enormous expenditure of health care resources. Survivors of respiratory failure face long-term consequences concerning their quality of life. New therapies are needed to improve early survival and to decrease long-term sequelae of this syndrome. The purpose of this study is to identify biological markers of disease in patients with ALI that are predictive of either disease susceptibility or prognosis, or that identify novel targets of therapeutic intervention.

DESIGN NARRATIVE:

As soon as possible after case identification, informed consent will be obtained from the patient or next of kin. Physiologic measurements and specimen collection will begin at the time of entry into the study. The inclusion criteria for this study allow entry of patients who have fulfilled criteria for ALI/ARDS for up to 48 hours. Bronchoalveolar lavage (BAL) fluid and blood will be collected at various times after the onset of ALI/ARDS in order to measure levels of a predetermined set of biological markers. In addition, DNA will be collected from patients and analysed for the presence of specific genetic polymorphisms that might alter either disease susceptibility or clinical expression of disease. The levels of these markers or the presence of specific genetic polymorphisms will be correlated with measure of pulmonary inflammation and extent of lung injury, as defined by: 1) PaO2/FiO2 ratios; 2) lung compliance; 3) plateau pressures; and 4) calculation of the Murray Lung Injury Score (obtained at entry and Days 1, 2, 3, 5, 7,10, 14, and 21). Secondary outcome measures to be directly correlated with biomarker expression will include indicators of maladaptive responses to ALI (including the development of multiple organ dysfunction syndrome [MODS]), fibroproliferation, and nosocomial pneumonia (events which greatly impact the clinical course of patients with ALI/ARDS). Thus, the secondary outcome measures include: 1) the development of organ failure (using the Sequential Organ Failure Assessment [SOFA] score); 2) time on ventilator; 3) ventilator-free days; 4) ICU and hospital length of stay; 5) hospital mortality; 6) development of pneumonia; 7) development of lung fibrosis (as determined by high-resolution computed tomography [HRCT] and pulmonary function testing); and 8) health related and lung-specific quality of life (as assessed with the Medical Outcome Studies 36-Item Short form Health Survey Standard Form [SF-36] and St. George's Respiratory Questionnaire).

Enrollment

40 patients

Sex

All

Ages

18 to 95 years old

Volunteers

No Healthy Volunteers

Inclusion criteria

Acute onset of illness with:

PaO2/FiO2 ratio of less than 300 (ALI) or PaO2/FiO2 ratio of less than 200 (ARDS)
Bilateral infiltrates consistent with pulmonary edema on frontal chest radiograph (infiltrates may be patchy, diffuse, homogeneous, or asymmetric)
Positive pressure ventilation via an endotracheal tube
No clinical evidence of left atrial hypertension (if measured, pulmonary arterial wedge pressure less than or equal to 18 mm Hg)
First three criteria must occur together within a 24-hour interval

Exclusion criteria

Greater than 48 hours elapsed following institution of mechanical ventilation
Pregnancy
Chronic respiratory failure as defined by any of the following:
1. FEV1 less than 20 ml/kg of PBW; FEV1/FVC less than 50%
2. Chronic hypercapnia or hypoxemia
3. Hospitalization within past 6 months for acute respiratory failure
4. Chronic home use of oxygen or mechanical ventilation
Left ventricular failure as defined by New York Heart Association (NYHA) class IV status
History of hematological malignancy or bone marrow transplantation
Entry in other intervention clinical trials
Decision of the patient or attending physician to forego aggressive care
Expected survival of less than 6 months (based solely on pre-existing medical problems [e.g., poorly controlled neoplasm or other end-stage disease])
AIDS (known history of HIV infection)
Prednisone (or equivalent) therapy of 20 mg/day or more for a period of at least 2 months with treatment continuing within 3 weeks prior to screening
Cytotoxic therapy within 3 weeks of screening
Morbid obesity defined as greater than 1 kg/c body weight
At risk for increased intracranial pressure that may result from permissive hypercapnia
Permissive hypercapnia that is contraindicated
Neuromuscular disease that would potentially impact ability to wean from mechanical ventilation
Receiving extracorporeal membrane oxygenation when meeting screening criteria