SIT LESS 4: How Does Reducing Sitting Time Improve Glucose and Lipid Metabolism? (SITLESS4)

Maastricht University Medical Centre (MUMC)

Status: Completed

Conditions: Overweight and Obesity

Treatments: Other: Activity regime

Study type: Interventional

Funder types: Other

Identifiers

NCT03912922

NL62413.068.17

Details and patient eligibility

About

Research has shown that replacing sitting time with low intensity physical activity (such as slowly walking and standing) has beneficial effects on metabolic health, like insulin sensitivity, comparable to improvements after sitting all day in combination with 1h streneous exercise.

The main objective of this study is to investigate the underlying mechanisms responsible for improved insulin sensitivity after 4 days of sitting less compared to sitting and exercise in healthy obese women. Our secondary objective is to investigate the effects of sitting less on cardio metabolic parameters.

Enrollment: 12 patients

Sex: Female

Ages: 45 to 70 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Signed informed consent
• Caucasian
• Postmenopausal women
• Aged 45-70 years at start of the study
• Body mass index (BMI) 25 - 35 kg/m2
• Stable dietary habits (no weight loss or gain >5kg in the past 3 months)
• Sedentary lifestyle (not more than 2,5 hours of exercise per week in the past 3 months)

Exclusion criteria

• Not able to complete the sitless try-out day according to the protocol
• Active diseases (cardiovascular, diabetes, liver, kidney, cancer or other)
• No use of medication interfering with investigated study parameters (as determined by responsible physician)
• Alcohol consumption of >2 servings per day
• Smoking in the past 6 months
• Participation in another biomedical trial which may have an effect on insulin sensitivity less than one month before the start of the study (screening visit)
• Mental or physical disability which interferes with physical activity
• Subjects with contra-indications for magnetic resonance imaging (MRI)
• Reported participation in night shift work 2 weeks prior to the start of the study (screening visit) and during study participation. Night work is defined as working between midnight and 6.00 AM.

Trial design

Primary purpose: Prevention

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: None (Open label)

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12 participants in 3 patient groups

Sit regime

Experimental group

Description:

Subjects will follow the sit regime during four days. Each day consists of 14 hours sitting, 1 hour walking and 1 hour standing and 8 hours sleeping.

Treatment:

Other: Activity regime

Sit less regime

Experimental group

Description:

Subjects will follow the sit less regime during four days. Each day will consist of 9 hours sitting, 3 hours walking, 4 hours standing and 8 hours sleeping.

Treatment:

Other: Activity regime

Exercise regime

Experimental group

Description:

Subjects will follow the exercise regime during four days. Each day will consist of 13 hours sitting, 1 hour walking, 1 hour standing, 1 hour exercise and 8 hours sleeping. The cycle session will be at approximately 60% maximal power. Exact cycling intensity and duration will be calculated to ensure equal total energy expenditure between the sitting less and the exercise regime.

Treatment:

Other: Activity regime