Skeletal Muscle and Adipose Tissue Responses After Acute Exercise: Comparing Effects of Three Different Intensities (3X)

University of Michigan

Status: Completed

Conditions: Changes Within Adipose Tissue in Response to Exercise at the Three Different Intensities; Relationship Between the Cellular Responses in Skeletal Muscle and the Changes in the Metabolomic Profile; Cellular Responses Within Skeletal Muscle in Response to Exercise of Different Intensities

Treatments: Behavioral: Low intensity exercise session; Behavioral: High intensity exercise session; Behavioral: Moderate intensity exercise session

Study type: Interventional

Funder types: Other

Identifiers

NCT05365334

HUM00204857

Details and patient eligibility

About

The study will assess the exercise-induced changes in chromatin accessibility and gene expression in a cell type-specific manner in skeletal muscle from healthy adults in response to exercise at three different intensities - 1) low-intensity continuous exercise, 2) moderate-intensity continuous exercise, and 3) high-intensity interval exercise.

Enrollment: 45 patients

Sex: All

Ages: 18 to 40 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Age:18-40
• Body Mass Index: 20-30 kg/m2
• Must engage in regularly planned aerobic-type exercise at moderate-vigorous intensity, 3 times per week for at least 30-60 minutes/session
• Women must have regularly occurring menses and must be premenopausal
• Subject willingness to opt-in for the Michigan Genomics Initiative (MGI) Repository

Exclusion criteria

• Evidence/history of cardiovascular or metabolic disease.
• Taking anti-coagulate medications or medications known to affect metabolism or inflammation
• Weight instability ≥ ±3kg in the last 6 months
• Tobacco or e-cigarette users
• Women must not be pregnant or actively lactating

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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45 participants in 3 patient groups

low-intensity continuous exercise

Experimental group

Description:

subjects will exercise at 30% maximal oxygen consumption (VO2max) for 45-60 minutes

Treatment:

Behavioral: Low intensity exercise session

moderate-intensity continuous exercise

Experimental group

Description:

subjects will exercise at 65% maximal oxygen consumption (VO2max) for 30-45 minutes

Treatment:

Behavioral: Moderate intensity exercise session

high-intensity interval exercise

Experimental group

Description:

subjects will completed 10x1minute exercise intervals at maximal oxygen consumption (VO2max) with 1 minute active recovery between

Treatment:

Behavioral: High intensity exercise session