Skeletal Muscle Protein Metabolism and Insulin Sensitivity in Overweight Individuals: Effects of Meals With Various Fatty Acid Compositions

University of Nottingham

Status: Completed

Conditions: Insulin Resistance

Treatments: Dietary Supplement: Placebo; Dietary Supplement: Saturated fat; Dietary Supplement: Fish Oil

Study type: Interventional

Funder types: Other

Identifiers

NCT03146286

SCA/OF/12/15

Details and patient eligibility

About

The aim of the study will investigate whether impairment in the action of insulin to promote the use of glucose in skeletal muscle (insulin resistance) as a result of oral ingestion of a liquid meal rich in saturated fat is linked to reduced ability of muscles to synthesise new protein in response to dietary protein intake, which ultimately may compromise maintenance of muscle size and quality of life and whether partially replacing saturated fat in the liquid meal with omega 3 polyunsaturated FA (n3PUFA) will ameliorate these negative effects.

Enrollment: 8 patients

Sex: Male

Ages: 40 to 60 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Male, healthy, overweight (BMI>27 kg/m2 and fat mass >30%) non-diabetic individuals.

Exclusion criteria

• Diabetes, smoking, cardiovascular disease

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Single Blind

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8 participants in 3 patient groups, including a placebo group

Control

Placebo Comparator group

Description:

Chocolate flavoured drink spiked with 15mg/kg \[2H31\]palmitate (D31palmitate) and singly-labelled water (H218O). 4 hours post test meal a bolus of milk protein (30 g), which has been intrinsically labelled with \[13C\]phenylalanine, along with 75 g of glucose in 250 ml of water will be given.

Treatment:

Dietary Supplement: Placebo

Saturated Fat

Experimental group

Description:

Chocolate flavoured drink spiked with 15mg/kg \[2H31\]palmitate (D31palmitate) and singly-labelled water (H218O) containing 0.7 g/kg bodyweight palm stearin. 4 hours post test meal a bolus of milk protein (30 g), which has been intrinsically labelled with \[13C\]phenylalanine, along with 75 g of glucose in 250 ml of water will be given.

Treatment:

Dietary Supplement: Saturated fat

Fish Oil

Experimental group

Description:

Chocolate flavoured drink spiked with 15mg/kg \[2H31\]palmitate (D31palmitate) and singly-labelled water (H218O) containing 0.45 g/kg bodyweight palm stearin and 0.25 g/kg bodyweight fish oil (n3PUFA). 4 hours post test meal a bolus of milk protein (30 g), which has been intrinsically labelled with \[13C\]phenylalanine, along with 75 g of glucose in 250 ml of water will be given.

Treatment:

Dietary Supplement: Fish Oil