Smoker Extracellular Vesicles Influence on Human Bronchial Epithelial Cells (Aerotox-1)

Lille Catholic University

Status: Completed

Conditions: Human Bronchial Epithelial Cells; Lung Pathogenesis; Smokers; Biomarkers

Treatments: Diagnostic Test: Broncho Alveolar Lavages (BAL)

Study type: Interventional

Funder types: Other

Identifiers

NCT03608293

RC-P0014

Details and patient eligibility

About

Cigarette smoking is a habit that has spread all over the world and is a significant risk factor for many diseases including cardiovascular disease, chronic obstructive pulmonary disease(COPD),asthma and lung cancer.

Evaluation and understanding of tobacco health effects are of major interest worldwide and answer to important societal concerns.

Identification of new biomarkers of exposure to tobacco smoke potentially implicated in COPD or lung carcinogenesis would allow a better observation of tobacco exposed population, thanks to screening establishment at reversible stages of pathological processes.

In this study, we question whether cigarettes smoking alters miRNA profiles of extracellular vesicles (EVs) present in human broncho alveolar lavages (BALs), which could affect surrounding normal bronchial epithelial cells status.

Full description

Extracellular vesicles (EVs) include a variety of nanoscale membranous vesicles (exosomes, microvesicles, microparticles). EVs are released into the interstitial fluid from a wide variety of normal or diseased cells.

Analysis of EVs and their content maybe useful as disease biomarkers as they reflect the contents of cells of origin, differ between normal and diseased tissue and can be reliably detected.

EVs may thus act as biomarkers of diverse pathologies like cancer, and detection of these biomarkers maybe applied to early diagnosis or assessment of prognosis in patients with cancer. EVs indeed contain both mRNAs and non-coding RNAs, such as small regulatory microRNAs (miRNAs).

Enrollment: 20 patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• >18 year-old

Exclusion criteria

• Lack of informed consent
• Drug treatment
• Professional exposure
• Evolutive pregnancy
• Bradycardia
• Respiratory assistance required,
• Diagnosed respiratory distress (e.g. COPD, asthma)
• Infectious pneumopathy
• Bronchial cancer

Trial design

Primary purpose: Basic Science

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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20 participants in 2 patient groups

Smokers

Experimental group

Description:

Smokers outpatients at the Pneumology service from the HôpitalSaint-Philibert (Lomme, France) to whom a bronchoalveolar lavage will be performed

Treatment:

Diagnostic Test: Broncho Alveolar Lavages (BAL)

Non smokers

Active Comparator group

Description:

Non smokers outpatients at the Pneumology service from the HôpitalSaint-Philibert (Lomme, France) to whom a bronchoalveolar lavage will be performed

Treatment:

Diagnostic Test: Broncho Alveolar Lavages (BAL)