Smoking and Peptidoglycan Recognition Protein-1 and Initial Periodontal Therapy

Kırıkkale University

Status: Completed

Conditions: Smoking, Cigarette

Treatments: Other: Initial periodontal therapy

Study type: Interventional

Funder types: Other

Identifiers

NCT03915730

2016/026

Details and patient eligibility

About

A total of 40 patients with CP (20 smokers (S+CP) and 20 nonsmokers (S-CP)) and 40 periodontally healthy subjects (20 smokers (S+PH) and 20 nonsmokers (S-PH)), comprising 80 subjects, were included in this study. Baseline GCF samples were obtained from all subjects and clinical periodontal measurements, including probing depth (PD), clinical attachment level (CAL), plaque index (PI), and gingival index (GI), were recorded. In patients who had received initial periodontal therapy, GCF samples were obtained and all clinical periodontal measurements were recorded again during the 6th-8th weeks. GCF PGRP-1 levels were analyzed by enzyme-linked immunosorbent assay.

Full description

Peptidoglycan recognition proteins (PGRPs) are one of the antimicrobial peptides that play a role in innate immunity and act against the bacterial cell wall. Smoking is the most important environmental risk factor for periodontitis. This study determined the effect of smoking on PGRP-1 levels in gingival crevicular fluid (GCF) in smokers and nonsmokers with chronic periodontitis (CP) following initial periodontal therapy.

Enrollment: 80 patients

Sex

Ages: 22 to 56 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Clinical diagnosis of severe chronic periodontitis
• Clinical diagnosis of periodontal health

Exclusion criteria

• have any systemic disease,
• to be alcohol consumer,
• to receive any periodontal therapy in the past 6 months,
• to take any drugs regularly
• to be pregnant and/or lactate.

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

---

80 participants in 4 patient groups

Smokers with chronic periodontitis

Experimental group

Description:

Patients who had teeth with 30% periodontal bone loss, presence of at least two nonadjacent sites per quadrant with probing pocket depths of ≥5 mm, and bleeding on probing were assigned as chronic periodontitis group Patients who have smoked for at least 10 years and a minimum of 10 cigarettes per day were assigned as current smokers.

Treatment:

Other: Initial periodontal therapy

Non-smokers with chronic periodontitis

Experimental group

Description:

Patients who had teeth with 30% periodontal bone loss, presence of at least two nonadjacent sites per quadrant with probing pocket depths of ≥5 mm, and bleeding on probing were assigned as chronic periodontitis group Never smoked patients were included into the nonsmoker group

Treatment:

Other: Initial periodontal therapy

Smokers with periodontal healthy

No Intervention group

Description:

Individuals were diagnosed periodontally healthy as without attachment loss, periodontal disease history, whole mouth bleeding scores of \<10% and a probing depth of ≤3 mm Patients who have smoked for at least 10 years and a minimum of 10 cigarettes per day were assigned as current smokers.

Non-smokers with periodontal healthy

No Intervention group

Description:

Individuals were diagnosed periodontally healthy as without attachment loss, periodontal disease history, whole mouth bleeding scores of \<10% and a probing depth of ≤3 mm Never smoked patients were included into the nonsmoker group