Sperm DNA Fragmentation Effects on Blastocyst Morphology Grading, Pregnancy Rate and Implantation Rate

Ganin Fertility Center

Status: Completed

Conditions: Blastocyst; Implantation Rate

Treatments: Procedure: ICSI

Study type: Observational

Funder types: Other

Identifiers

NCT04144621

MH0001

Details and patient eligibility

About

Retrospective cohort study for 189 couples assigned to normal and abnormal Sperm DNA fragmentation (SDF) groups.To determine the effect of SDF on blastocyst's trophectoderm (TE), Inner cell mass (ICM) morphology grading, and implantation rate. Semen in both groups was processed by density gradient (DG).

Full description

Purpose: to determine if sperm DNA fragmentation (SDF) has a correlation with Blastocyst's trophectoderm (TE) and Inner cell mass (ICM) grading, or with the implantation rate?

Method: Retrospective cohort study for 189 couples, assigned to normal SDF group (121 cases), and abnormal SDF group (68 cases). Semen in both groups was processed by double layer density gradient (DG).

Statistical analysis done using SPSS version 22.

Enrollment: 189 patients

Sex: All

Ages: Under 37 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• All cases in this study have to reach the blastocyst stage.
• Female age ≤37 years old.
• Male sperm count ≥ 5 million.

Exclusion criteria

• Usage of sperm selection techniques.
• Testicular aspiration or testicular biopsies.
• Oocyte or sperm donation.

Trial design

189 participants in 2 patient groups

Normal SDF

Description:

Couples with male partners having SDF lower than 20% using TUNEL assay

Treatment:

Procedure: ICSI

Abnormal SDF

Description:

Couples with male partners having SDF greater than 20% using TUNEL assay

Treatment:

Procedure: ICSI