Sperm Selection and Embryo Development: Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods Sperm Selection and Embryo Development: Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods

Maltepe University

Status: Completed

Conditions: IVF; Reduced Sperm Motility; Astheno Teratozoospermia; ICSI

Treatments: Device: Microfluidic sperm sorting chip; Device: Density gradient

Study type: Interventional

Funder types: Other

Identifiers

NCT04962074

2019

Details and patient eligibility

About

Purpose: To compare the effects of the density gradient centrifugation and microfluidic chip sperm preparation methods on embryo development in a study population with astheno-teratozoospermia.

Materials and Methods: In this prospective study, the semen samples of the patients with astheno-teratozoospermia were divided into two groups for preparation with either the microfluidic or density gradient methods. Selected spermatozoa were used to fertilize the mature sibling oocytes. Semen parameters and embryo development on days 3 and 5 were assessed.

Enrollment: 22 patients

Sex: Male

Volunteers: No Healthy Volunteers

Inclusion criteria

• Infertile men with astheno-teratozoospermia
• Women were aged from 18 to 39
• Women had at least two mature oocytes.

Exclusion criteria

• MI oocytes

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Single Blind

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22 participants in 2 patient groups

Density gradient method

Experimental group

Description:

Sperm prepared by density gradient method

Treatment:

Device: Density gradient

Microfluidic chip

Experimental group

Description:

Sperm prepared by microfluidic chip method

Treatment:

Device: Microfluidic sperm sorting chip