Spermatogonial Stem Cells in Azoospermic Patients: a Comparison Between Obstructive and Non-obstructive Azoospermia

Hillel Yaffe Medical Center

Status: Unknown

Conditions: Male Infertility

Treatments: Other: Biospecimen staining

Study type: Observational

Funder types: Other

Identifiers

NCT01178463

HYMC-0065-09

Details and patient eligibility

About

The researchers hypothesized that nonobstructive azoospermia might be associated with a reduction OF SSCs in seminiferous tubules as compared with obstructive azoospermia. Testicular sperm specimens that had been previously extracted from azoospermic patients will be paraffin embedded and stained with anti GPR-125. After antigen retrieval, the sections will be incubated with rabbit anti GPR125 followed by Cy3-conjugated anti rabbit secondary antibodies. Those found to be SSCs will be counted using light microscopy, and compared between patients with obstructive (n=11) and non-obstructive azoospermia (n=9).

Enrollment: 25 estimated patients

Sex: Male

Ages: 18+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Azoospermic patients

Exclusion criteria

• Patients with sperm cells

Trial design

25 participants in 2 patient groups

I. Obstructive Azoospermia

Treatment:

Other: Biospecimen staining

Other: Biospecimen staining

II. Non-Obstructive Azoospermia Patients

Treatment:

Other: Biospecimen staining

Other: Biospecimen staining