Stem Cell Recruitment in Exercised Skeletal Muscles of Older Women by Phytosteroid Rg1

University of Taipei

Status: Completed

Conditions: Exercise Training; Skeletal Muscle Recovery; Bone Marrow Stem Cells; Post-menopausal Women

Treatments: Dietary Supplement: exercise following Rg1 consumption; Dietary Supplement: Placebo

Study type: Interventional

Funder types: Other

Identifiers

NCT07093190

13A1-CG-CO-05-2426-3 (Other Grant/Funding Number)

NSTC 110-2622-H-845-004 (Other Grant/Funding Number)

IRB-2021-045

Details and patient eligibility

About

The goal of this interventional study is to observe the effects of bone marrow stem cell behavior in skeletal muscle of women between 60 to 75 years old after a single moderate resistance exercise session. Plant sterol Rg1, a compound known to enhance stem cell infiltration following exercise in young men will be tested against a placebo in a double blinded crossover design.

The main questions to be answered are:

• Does Rg1 supplementation alter serum sex hormone levels in recovery from exercise?
• Does Rg1 supplementation influence bone marrow stem cell differentiation in skeletal muscles of older women?

Participants will:

• Pre-test body composition, maximal leg strength, blood and muscle tissue.
• One hour after taking the supplement, participants will perform 4 sets of 10 repetitions of 70% of maximal strength (1RM) leg press and have two thigh muscle biopsies (0 and 24 h after exercise).
• Repeat the protocol one month later on the alternative supplement. Researchers will understand whether Rg1 improves recovery response to resistance exercise in post-menopausal women.

Enrollment: 11 patients

Sex: Female

Ages: 60 to 70 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• sedentary, able to cease medications and supplements for 48 h before and 24 h after exercise session, medically sound for lower body moderate resistance exercise

Exclusion criteria

• use of insulin, sex hormone and immune-modulating medications, smoking and regular alcohol consumption

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Triple Blind

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11 participants in 2 patient groups, including a placebo group

exercise following Rg1 consumption

Experimental group

Description:

1 hour following the consumption of the Rg1 supplement, participants performed exercise intervention before muscle biopsies were taken.

Treatment:

Dietary Supplement: exercise following Rg1 consumption

exercise only following placebo consumption

Placebo Comparator group

Description:

Crossover trial with placebo supplementation consumed before exercise

Treatment:

Dietary Supplement: Placebo