Study of Congenital Orofacial Clefts by Implementing Optical Genome Mapping (CARTOFENTE)

Centre Hospitalier Universitaire, Amiens

Status

Enrolling

Conditions

Optical Genome Mapping

Orofacial Clefts

Next Generation Sequencing (NGS)

Treatments

Genetic: blood withdrawal

Study type

Interventional

Funder types

Other

Identifiers

NCT06880094

PI2023_843_0023

Details and patient eligibility

About

Orofacial clefts, the most common congenital craniofacial malformations, have a complex etiology involving an interaction between genetic and environmental factors.

Chromosomal abnormalities, including structural variations, represent a major cause of human pathology. Recently, technological developments and the introduction of next-generation sequencing (NGS) technologies have revolutionized the field of medical genetics.

Optical genome mapping (OGM) is an innovative, high-resolution "long read" technique that enables the identification of all classes of chromosomal variation, consisting in the direct visualization of long, labeled DNA molecules throughout the genome. This technology is gradually becoming an essential tool for studying onco-hematology and constitutional genetic pathologies The purpose of this study is to search for structural chromosomal variants (SV) or copy number variants (CNV) not identifiable either by cytogenetic methods nor by "short read" NGS "short read, in individuals with oral-facial clefts with no genetic diagnosis.

Enrollment

26 estimated patients

Sex

All

Volunteers

No Healthy Volunteers

Inclusion criteria

Individuals with syndromic, complex or familial oral-facial clefts
With no established genetic diagnosis
Followed up at the Amiens-Picardie University Hospital

Exclusion criteria

genetic diagnosis of oral-facial cleft
No health insurance affiliation
Patient under guardianship or curatorship, under safeguard of justice or deprived under public law
Pregnant, parturient or breast-feeding woman