Study of Synapsis and Recombination in Male Meiosis and the Implications in Infertility

Igenomix

Status: Completed

Conditions: Azoospermia; Abnormal Spermatogenesis

Treatments: Other: non interventional

Study type: Observational

Funder types: Other; Industry

Identifiers

NCT02339272

070-C-027-CR

Details and patient eligibility

About

The aim of this study was to assess meiotic recombination in primary spermatocytes, synaptonemal complex length and the correlation with chromosomal abnormalities in testicular spermatozoa from infertile men with idiopathic non-obstructive azoospermia (NOA).

Full description

During the first meiotic division in spermatogenesis there are two critical events. First, synapsis between homologous chromosomes and formation of the synaptonemal complex (SC), which regulates sister chromatid cohesion and provides the template for localization of recombination machinery proteins. Secondly, recombination between homologous chromosomes, which is essential for the correct segregation. Errors during these two processes may induce incorrect segregation of chromosomes and are a major cause of gamete aneuploidy. The aim of this study was to assess meiotic recombination in primary spermatocytes, synaptonemal complex length and the correlation with chromosomal abnormalities in testicular spermatozoa from infertile men with idiopathic non-obstructive azoospermia (NOA). Prospective cohort study to assess meiotic progression, total length of SC, frequency of recombination and sperm aneuploidy in samples obtained from testicular biopsies from NOA patients. The study group was compared with a control group from post-vasectomized (OA) patients. Immunocytogenetics with SCYP3, CREST and MLH1 antibodies for meiotic progression, SC length, and recombination. Fluorescence in situ Hybridization (FISH) for chromosomes 1, 4, 6, 13, 16, 18, 21, 22 in primary spermatocytes and chromosomes 13, 18, 21, X, Y on sperm. MicroMeasure 3.3 program was used for SC length.

Enrollment: 50 patients

Sex: Male

Volunteers: No Healthy Volunteers

Inclusion criteria

• NOA patients undergoing ICSI with testicular sperm
• OA post-vasectomized patients undergoing ICSI with testicular sperm

Exclusion criteria

• Abnormal Karyotype

Trial design

50 participants in 2 patient groups

NOA

Description:

Infertile males with non obstructive azoospermia

Treatment:

Other: non interventional

OA

Description:

Post-vasectomized patients with proven fertility before vasectomy

Treatment:

Other: non interventional