Study of the Expression of Met Receptor Fragments in Non Small Cell Lung Cancers (MetLung)

Centre Oscar Lambret

Status

Completed

Conditions

Non Small Cell Lung Cancer

Treatments

Other: Met analysis on tissue and blood

Study type

Interventional

Funder types

Other

Identifiers

NCT01647867

MetLung - 1204

Details and patient eligibility

About

Our project, established through collaboration between clinicians (Oscar Lambret Center-University Hospital) and scientists (IBL), aims to evaluate the expression of Met fragments in the lung cancer (LC). Unlike previous studies on Met by sequencing, in situ hybridization or immunohistochemistry, the investigators propose a protein analysis by Western blot of tumor samples and healthy tissue. This approach will evaluate the expression of full-length receptor, the potential presence of intracellular fragments, and their phosphorylation status.

Full description

Patient information and collection of signed inform consent
Blood sample collection before bronchoscopy or before surgery, at the same time as a usual blood assessment.
Prospective collection of bronchial biopsies of patients with metastatic non small cell lung cancer, during a bronchoscopy at the Oscar Lambret Center (Dr Dansin)
Prospective collection of resected specimens of patients with localized non small cell lung cancer, who were operated at the thoracic surgery unit of the University hospital (Pr Porte)
Transportation of the samples to the pathology laboratories (Dr Robin, Pr Copin)
Selection of blocks of interest and preparation of tumor samples
Clinical data collection : date of biopsy or surgery, disease-free survival, overall survival, tumor characteristics, EGFR status, ALK, BRAF, HER2
Transportation of tumor blocks and blood samples to the Institute of Biology of Lille for analyses: Western blot, immunohistochemistry and ELISA test)
Return to storage of the tumor samples at the Oscar Lambret Center and at the University hospital of Lille

Enrollment

56 patients

Sex

All

Ages

18+ years old

Volunteers

No Healthy Volunteers

Inclusion criteria

Patients with non small cell lung cancer (NSCLC)
Patients candidate for bronchial biopsies at the Oscar Lambret Center (metastatic stage) or candidate for thoracic surgery at University Hospital of Lille (localized stage)
Treatment naive patient for metastatic NSCLC
Patients with or without neoadjuvant therapy for localized NSCLC
Male or female patients aged ≥ 18 years
Patient registered with the social security system
Having signed a written Informed Consent

Exclusion criteria