Study of the T CD8 Immune Response in Horton's Disease (Horton CD8)

University Hospital Center (CHU) Dijon Bourgogne

Status

Completed

Conditions

Horton's Disease

Treatments

Other: Blood sample drawn into a 5 mL dry tube

Other: Blood sample drawn into a 5 mL dry tube at the diagnosis and after 3 months of treatment

Other: 16 blood samples drawn into 6 mL heparinized tubes at the diagnosis and after 3 months of treatment

Other: 16 blood samples drawn into 6 mL heparinized tubes

Study type

Observational

Funder types

Other

Identifiers

NCT02158208

SAMSON APJ 2013

Details and patient eligibility

About

The research hypothesis is that T lymphocytes CD8 play a role in the physiopathology of Horton's disease.

At the inclusion visit, patients will have, as is the case in the usual strategy:

A complete clinical examination carried out by the doctor in charge of the patient
ESR, and CRP and fibrinogen assay
A full blood count for leukocytes and lymphocytes
A biopsy of the temporal artery (TAB) to screen for signs of vascularitis, suggesting Horton's disease. The clinician in charge of the patient will decide if a second biopsy is necessary. The biopsy will be sent to and analysed at Anatomy and Pathological cytology service. Immunohistochemical analyses will be done if the TAB is positive.

In addition to the standard clinical examination and complementary examinations relative to the patients' pathology, the following will be done:

Lymphocyte immunophenotyping for the quantity of T CD4 (cluster of differentiation 4) and CD8 lymphocytes, B lymphocytes and natural killer lymphocytes. This will make it possible to calculate the absolute value for different T lymphocyte populations.
A blood sample drawn into a dry 5 mL tube (large yellow) to isolate the serum, which will be stored at -80°C for future assays for cytokines and other biomarkers of interest for Horton's disease.
16 blood samples drawn into 6 mL heparinized tubes (large green). These will be used immediately for cytometric and functional analyses.

Enrollment

30 patients

Sex

All

Ages

50+ years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

Patients

Patients who have provided written informed consent
Patients covered by national health insurance
Age > 50 years
Patients with Horton's disease at the diagnosis before any treatment

Horton's disease is defined by American College of Rheumatology criteria, the diagnosis is made if any 3 of the following 5 criteria are associated:

age at the onset of the disease 50 years or above
recent onset localised headache
indurated temporal artery or decrease/absence of temporal pulse
erythrocyte sedimentation rate (ESR) above 50 mm for the first hour (or CRP>20 mg/L)
positive TAB showing vascularitis with infiltration of mononucleated cells or granulomatous inflammation with or without giant cells.

Controls Controls will be healthy volunteers recruited from blood donors of Dijon CHU, voluntary hospital personnel (nurses, doctors, laboratory technicians and secretaries) and patients without infectious or inflammatory disease, cancer or auto-immune disease (CRP<5mg/L) recruited in the department of the investigators at Dijon CHU. They will be matched for age and sex.

Age > 50 years
Patients covered by national health insurance
who have provided written informed consent to take part
Absence of inflammatory syndrome (CRP<5 mg /L)

Exclusion criteria

Any patient not meeting inclusion criteria
Patient treated with corticoids or immunosuppressants in the month preceding inclusion
Patients treated with chemotherapy