Study on the Efficacy and Safety of the TmBU Conditioning Regimen in High-risk or Relapsed/Refractory Acute Leukemia

Soochow University

Status and phase: Enrolling; Phase 2

Conditions: Conditioning Therapy; Transplantation, Stem Cell; Acute Lymphoblastic Leukemia; Acute Myeloid Leukemia

Treatments: Drug: TmBU conditioning Regimen; Drug: mBUCY conditioning regimen

Study type: Interventional

Funder types: Other

Identifiers

NCT06816134

SZConditioning01

Details and patient eligibility

About

This project is a prospective, single-center, randomized controlled clinical study. The subjects were high-risk or relapsed/refractory AML or ALL patients aged ≤ 65 years diagnosed by bone marrow cell morphology, immunology, genetics and therapeutic efficacy evaluation. The TmBU scheme or modified Bu/Cy (mBuCy) scheme was used for pretreatment in allo-HSCT. The primary endpoint of the study was the 2-year cumulative incidence of relapse (CIR) after allo-HSCT, and the secondary endpoints were 2-year overall survival rate (OS), progressing-free survival rate (PFS), non-relapse mortality rate (NRM), graft-versus-host disease (GVHD)-free relapse-free survival (GRFS) rate.

Enrollment: 48 estimated patients

Sex: All

Ages: 15 to 65 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Confirmed diagnosis of AML or ALL according to WHO 2022 guideline criteria, with indications for allo-HSCT list below:

  1. Relapsed/primary refractory (definitions refer to NCCN 2025) or genetic high-risk group AML at diagnosis (risk stratification refers to ELN 2022)
  2. High-risk at diagnosis (risk stratification refers to ELN 2022) or MRD positive before transplantation B-ALL
  3. Confirmed diagnosis of T-ALL
  4. History of central nervous system leukemia (CNSL) or histopathologically confirmed extramedullary manifestation (EMD) during the course of the AML or ALL

• Age 15-65 years old (≤ 65 years old)

• HCT-CI score < 2 points ECOG 0-2 points

• Adequate organ function:

  1. Cardiac NYHA grade ≤ 2, left ventricular ejection fraction ≥55%
  2. Creatinine clearance ≥ 50ml/min
  3. ALT and AST ≤ 2.5 times the upper limit of the normal range, and total bilirubin ≤ 1.5 times the upper limit of the normal range
  4. Oxygen saturation > 92% without oxygen

• Expected survival time ≥ 3 months

• Ability to understand and voluntarily sign the informed consent form

Exclusion criteria

• With other malignant tumors and have received any treatment for this tumor within the past 3 years
• Previous or current other CNS disease (such as epilepsy, generalized seizure disorder, paresis, aphasia, stroke, severe brain injuries, dementia, Parkinson's disease, cerebellar disease, organic brain syndrome, or psychosis) or any CNS-related autoimmune disease
• HIV/Syphilis infection or uncontrolled active other infections (bacteria or fungus or virus is included)
• With active hepatitis B or hepatitis C infection
• Patients received cardiac angioplasty or stent implantation within 12 months before signing the informed consent form, or have symptoms requiring medical treatment for coronary heart disease
• With primary immunodeficiency or active autoimmune disease
• Previous history of severe immediate hypersensitivity reactions to any of the drugs to be used in this study
• Received a live vaccine within 6 weeks prior to screening
• Pregnant, lactating females and patients of childbearing potential who are unwilling to use contraception
• Inability to cooperate with the requirements of study, treatment and monitoring due to psychiatric illness or other conditions
• Patients not suitable for the study according to the investigator's assessment

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

---

48 participants in 2 patient groups

TmBu conditioning Regimen Group

Experimental group

Description:

TmBU conditioning regimen（TT 9mg/kg -8\~-7d; Ara-C 2g/m2/d -6d; CDA 5 mg/m2/d -6d; Bu 3.2 mg/kg/d from -5 to -3 days）

Treatment:

Drug: TmBU conditioning Regimen

mBUCY conditioning Regimen Group

Active Comparator group

Description:

mBuCy conditioning regimen（CCNU/BCNU -9d, Ara-C 2g/m2/d -8d; CDA 5 mg/m2/d -8d; Busulfan 3.2 mg/kg/d from -7 to -5 days; Cyclophosphamide 1.8 mg/m2/d from -4 to -3 days）

Treatment:

Drug: mBUCY conditioning regimen