Studying Biomarkers in Cell Samples From Patients With Acute Myeloid Leukemia

Children's Oncology Group

Status

Completed

Conditions

Leukemia

Treatments

Genetic: protein expression analysis

Genetic: RNA analysis

Other: laboratory biomarker analysis

Genetic: gene expression analysis

Other: fluorescence activated cell sorting

Genetic: mutation analysis

Study type

Observational

Funder types

NETWORK

NIH

Identifiers

NCT01057199

CDR0000664229 (Other Identifier)

COG-AAML10B13 (Other Identifier)

NCI-2011-02207 (Registry Identifier)

AAML10B13

Details and patient eligibility

About

RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.

PURPOSE: This research study is looking at biomarkers in cell samples from patients with acute myeloid leukemia.

Full description

OBJECTIVES:

To determine the significance of regulation of microRNA-34a and microRNA-194 by C/EBPα during granulopoiesis in patients with acute myeloid leukemia with CEBPA mutations.
To determine whether overexpression of micro RNA-34a and microRNA-194 in myeloid progenitors with C/EBPα mutations leads to granulocytic differentiation.
To examine how microRNA-34a and microRNA-194 downregulate E2F3 during granulopoiesis.
To determine the role of microRNA-34a and microRNA-194 in myeloid cell proliferation.

OUTLINE: Cryopreserved cell samples are collected for laboratory analysis, including mutation analysis, gene expression analysis, RNA analysis, and fluorescence-activated cell sorting (FACS) analysis.

Enrollment

6 patients

Sex

All

Volunteers

No Healthy Volunteers

Inclusion and exclusion criteria

DISEASE CHARACTERISTICS: