Studying Gene Expression in Tissue Samples From Patients With Acute Myeloid Leukemia

Children's Oncology Group

Status: Completed

Conditions: Leukemia

Treatments: Other: laboratory biomarker analysis; Genetic: reverse transcriptase-polymerase chain reaction; Genetic: RNA analysis; Genetic: gene expression analysis

Study type: Observational

Funder types: NETWORK; NIH

Identifiers

NCT01057095

NCI-2011-02208 (Registry Identifier)

CDR0000664240 (Other Identifier)

AAML10B12

COG-AAML10B12 (Other Identifier)

Details and patient eligibility

About

RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.

PURPOSE: This research study is looking at gene expression in tissue samples from patients with acute myeloid leukemia.

Full description

OBJECTIVES:

• Screen acute myeloid leukemia (AML) samples to correlate high relative expression levels of PLAGL2 and putative PLAGL2 target genes (implicated in microarray study) with potential pathogenic relevance with respect to AML development.

OUTLINE: Cryopreserved mRNA from diagnostic samples is analyzed for gene expression by reverse transcriptase-PCR.

Enrollment: 20 patients

Sex: All

Ages: Under 120 years old

Volunteers: No Healthy Volunteers

Inclusion and exclusion criteria

DISEASE CHARACTERISTICS:

• Diagnosis of acute myeloid leukemia, including inv(16)(p13;q22) abnormalities

  • No t(15;17)(q22;q12) or t(8;21)(q22;q22) abnormalities

PATIENT CHARACTERISTICS:

• Not specified

PRIOR CONCURRENT THERAPY:

• Not specified