Studying Genes in Tissue Samples From Younger and Adolescent Patients With Soft Tissue Sarcomas

Children's Oncology Group

Status: Completed

Conditions: Childhood Angiosarcoma; Childhood Liposarcoma; Childhood Neurofibrosarcoma; Childhood Epithelioid Sarcoma; Metastatic Childhood Soft Tissue Sarcoma; Recurrent Childhood Soft Tissue Sarcoma; Nonmetastatic Childhood Soft Tissue Sarcoma; Chordoma; Childhood Alveolar Soft-part Sarcoma; Childhood Leiomyosarcoma; Childhood Synovial Sarcoma; Childhood Desmoplastic Small Round Cell Tumor; Childhood Malignant Mesenchymoma; Childhood Fibrosarcoma; Desmoid Tumor

Treatments: Other: laboratory biomarker analysis

Study type: Observational

Funder types: NETWORK; NIH

Identifiers

NCT01567046

NCI-2012-00714 (Registry Identifier)

ARST12B5

Details and patient eligibility

About

This research trial studies genes in tissue samples from younger and adolescent patients with soft tissue sarcomas. Studying samples of tumor tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. It may also help doctors find better ways to treat cancer

Full description

Study Subtype: Ancillary/Correlative Observational Study Model: Cohort Time Perspective: Retrospective Biospecimen Retention: Samples With DNA Biospecimen Description: Tissue Study Population Description: Existing NRSTS samples from the COG D9902/ARST0332 studies Sampling Method: Non-Probability Sample

OBJECTIVES:

I. To determine the frequency with which actionable mutations are found in archived non-rhabdomyosarcoma soft tissue sarcoma (NRSTS) tumor specimens using mass spectrometry (MS) analysis of tumor-derived deoxyribonucleic acid (DNA).

OUTLINE:

Archived DNA tissue samples are analyzed for frequency of genetic mutations, including single nucleotide polymorphisms (SNPs), single nucleotide variants (SNVs), and small deletions and/or insertions, by polymerase chain reaction (PCR) and mass spectometry (Sequenom MassARRAY). Results are then analyzed to determine whether specific mutations correlate with patient or disease features such as tumor stage, histological grade, or outcome.

Enrollment: 70 patients

Sex: All

Ages: Under 30 years old

Volunteers: No Healthy Volunteers

Inclusion and exclusion criteria

Inclusion Criteria:

• Archived non-rhabdomyosarcoma soft tissue sarcoma (NRSTS) tumor-derived DNA

  • Synovial sarcoma, malignant peripheral nerve sheath tumor (MPNST), soft tissue sarcoma not otherwise specified (NOS), or other less common pediatric NRSTS

  • Formalin-fixed, paraffin-embedded (FFPE) tissue from patients enrolled on:

    • COG-D9902 Soft Tissue Sarcoma (STS) Biology and Banking Protocol
    • COG-ARST0332 A Risk-Based Treatment for Pediatric NRSTS Study

• See Disease Characteristics

Trial design

70 participants in 1 patient group

Correlative studies

Description:

Archived DNA tissue samples are analyzed for frequency of genetic mutations, including SNPs, SNVs, and small deletions and/or insertions, by PCR and mass spectometry (Sequenom MassARRAY). Results are then analyzed to determine whether specific mutations correlate with patient or disease features such as tumor stage, histological grade, or outcome.

Treatment:

Other: laboratory biomarker analysis