Studying Genes Using Cord Blood and Placenta Samples From Relatively Healthy Newborns and Samples From Younger Patients With Wilms Tumor

Children's Oncology Group

Status: Completed

Conditions: Kidney Cancer

Treatments: Genetic: polymorphism analysis; Other: laboratory biomarker analysis; Genetic: DNA methylation analysis; Genetic: allele-specific oligonucleotide real-time quantitative polymerase chain reaction; Genetic: gene expression analysis; Genetic: nucleic acid sequencing; Genetic: RNA analysis

Study type: Observational

Funder types: NETWORK; NIH

Identifiers

NCT01576211

NCI-2012-00726 (Registry Identifier)

CDR0000730596 (Other Identifier)

AREN12B6 (Other Identifier)

COG-AREN12B6 (Other Identifier)

Details and patient eligibility

About

RATIONALE: Studying samples of blood and tissue from newborns and from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.

PURPOSE: This research trial studies cord blood and placenta tissue from newborns, and tumor tissue samples from patients with Wilms tumor.

Full description

OBJECTIVES:

• What is the frequency of loss of imprinting at birth (in the cord blood and placenta) in a relatively healthy birth cohort?
• Does deoxyribonucleic acid (DNA) methylation levels at imprinting genes have a direct association to the gene expression?

OUTLINE: Archived tumor tissue, cord blood, and placenta samples are analyzed for DNA methylation, single nucleotide polymorphism, and gene expression by polymerase chain reaction (PCR), pyrosequencing, and quantitative real-time PCR. Information regarding gender and age of the samples are also collected, if possible.

Enrollment: 40 estimated patients

Sex: All

Ages: Under 21 years old

Volunteers: Accepts Healthy Volunteers

Inclusion and exclusion criteria

DISEASE CHARACTERISTICS:

• Wilms tumor tissue samples from Caucasians (fresh or frozen), or DNA and ribonucleic acid (RNA) samples already isolated from patients registered on Children's Oncology Group Wilms tumor protocols
• Normal/control blood samples from matched individuals
• Cord blood/ placenta samples from the Michels lab Epigenetic Birth Cohort

PATIENT CHARACTERISTICS:

• Not specified

PRIOR CONCURRENT THERAPY:

• Not specified