Studying Tumor Tissue Samples From Patients With Soft Tissue Sarcoma

University of Arizona

Status: Completed

Conditions: Sarcoma

Treatments: Genetic: western blotting; Genetic: gene expression analysis; Other: immunohistochemistry staining method; Genetic: reverse transcriptase-polymerase chain reaction; Genetic: protein expression analysis; Genetic: DNA analysis; Other: laboratory biomarker analysis

Study type: Observational

Funder types: Other; NIH

Identifiers

NCT00900341

P30CA023074 (U.S. NIH Grant/Contract)

05-0509-04

UARIZ-HSCA0535 (Other Identifier)

UARIZ-17879

Details and patient eligibility

About

RATIONALE: Studying samples of tumor tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.

PURPOSE: This research study is looking at tumor tissue samples from patients with soft tissue sarcoma.

Full description

OBJECTIVES:

• To develop a pilot program for the collection, archiving, and experimental analyses of soft tissue sarcomas (STS).
• To obtain STS tumors from cancer patients undergoing tumor removal or core needle biopsies.
• To evaluate cellular and molecular factors involved in STS oncogenesis by studying samples derived from human tumor specimens.
• To develop an accompanying bioinformatics database of the demographics of STS patients from which tumor specimens are derived for experimental study.

OUTLINE: Tumor tissue samples are analyzed for gene and protein expression by cDNA microarray analysis, RT-PCR, immunohistochemistry, in vitro transformation assays, RNA interference, and Western blot or immunoprecipitation.

Enrollment: 27 patients

Sex: All

Volunteers: No Healthy Volunteers

Inclusion and exclusion criteria

DISEASE CHARACTERISTICS:

• Meets one of the following criteria:

  • Patients with soft tissue sarcoma (STS) undergoing evaluation, treatment, and/or follow-up at the Arizona Cancer Center or University Medical Center
  • Patients undergoing surgical treatment for STS removal or core needle biopsy for medically indicated diagnostic or therapeutic purposes

PATIENT CHARACTERISTICS:

• Not specified

PRIOR CONCURRENT THERAPY:

• Not specified