Substrate Utilization and Hormonal Status in Women

University Hospital, Clermont-Ferrand

Status: Completed

Conditions: Substrate Utilization

Study type: Observational

Funder types: Other

Identifiers

NCT01095614

CHU-0068

AU818 (Other Identifier)

Details and patient eligibility

About

Women sexual hormones (i.e. estrogen) are involved in the regulation of substrate utilization and storage. Being under oral contraception (OC) may conduct to metabolic and hormonal modifications, but results are confused regarding the impact on substrate utilization. In the present study, the aim is then to explore the effect of hormonal status on substrate utilization during sous-maximal exercise, comparing women with and without OC.

We hypothesised that women under OC have a lower Respiratory Exchange Ratio (RER) at exercise than women without contraception underlying a greater reliance on fat oxidation.

Full description

24 healthy young women (12 with OC and 12 without any contraception) will complete a maximal test on an ergometer cycle to obtain their maximal oxygen consumption (VO2max).Their body composition will be assessed by DXA. They will be asked to complete 3 experimental sessions.

During a first session the volunteers will remain inactive, and only a breakfast will be offered. During the second session, a breakfast will be offered and they will have to complete a sub-maximal exercise set at 60% of their VO2max 3 hours after breakfast. And the third session will consist in the realisation of exercise on fast state. Those three sessions will be realized in a randomized order. Several blood (catheter) and salivary samples will be collected during the three sessions, throughout the experimental day.

Enrollment: 24 estimated patients

Sex: Female

Ages: 19 to 30 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Female
• Adults between 19 to 30 years old
• Body mass index between 20 and 25 kg.m-²
• Oral contraception (half of the sample) : mini-dosed, and mono-phasic, ethinyl estradiol ≤ 30 µg
• Without contraception (half of the sample)
• Affiliated to National Health Insurance
• Subject giving her written informed consent
• Subject considered as normal after clinical examination and medical questionnaire.

Exclusion criteria

• Chronic pathologies : cardiovascular diseases, cancer, chronic inflammation diseases, renal, intestinal impairments
• Refusal to be registered on the National Volunteers Data file
• Being in exclusion on the National Volunteers Data file
• Practising intensive physical exercise
• Heavy consumer of alcohol or/and tobacco
• Previous medical and/or surgery judged by the investigator as incompatible with this study
• Pregnancy

Trial design

24 participants in 2 patient groups

12 women with oral contraception

12 women without any contraception