T Cell Responses to Various Levels of Exercise Stress

Kansas Board of Regents

Status: Completed

Conditions: Healthy

Treatments: Other: Exercise

Study type: Interventional

Funder types: Other

Identifiers

NCT06638684

ONR N000141410202 (Other Grant/Funding Number)

STUDY00001969

Details and patient eligibility

About

The objective of this project is to quantify the immunoendocrine response to acute stress events combining both physical and psychological challenges. This work will serve as a pilot project examining differential stress induction in response to exercise. Participants will have an instructor with prior military training lead a two-hour training session for 10 recreationally fit male volunteers. You will be randomly assigned to a high-intensity training group to participate in the "military style" training and a low-intensity training group who will participate in a low-intensity cardiovascular training protocol. Blood samples will be collected from you prior to and following the exercise session and these samples will be analyzed for endocrine measures as well as markers of immune function to include chemokines and cytokines.

Enrollment: 16 patients

Sex: Male

Ages: 18 to 35 years old

Volunteers: Accepts Healthy Volunteers

Inclusion and exclusion criteria

All subjects will be screened using a health-history questionnaire for contradictions to exercise by American College of Sports Medicine (ACSM) guidelines

Inclusion Criteria:

• Recreationally active
• Between the ages of 18-40,
• Healthy
• Non-obese (BMI <28 kg/m2)
• Non-smoking
• Free of metabolic or cardiovascular diseases.

Exclusion Criteria:

• Unhealed Fractures
• Thrombophlebitis (blood clots)
• Recent Surgery (within the last three months)
• Recent Uncontrolled Bruising
• Osteomyelitis (acute or chronic bone infection)
• Myositis Ossificans (hardened scarring in muscle tissue of the thigh).

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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16 participants in 2 patient groups

Moderate intensity exercise

Active Comparator group

Description:

Subjects will participate in a 100 min bout of exercise led by an exercise physiologist doing the same exercises as the high-intensity group, but at a lower effort level. Exercise consists of of a mix of running activities (≤ 400 m); body weight exercises, such as burpees and lunges; and exercises, such as crunches and squats utilizing 20.4 kg weight plates, but performed at a pace allowing for in-session recovery. Scheduled 5 min rests will be included at 30, 60, and 85 min and MI subjects will be similarly encouraged to consume water and/or the carbohydrate/electrolyte drink. The researchers will obtain a blood sample from you prior to and immediately following the exercise, as well as one, four and six hours post-exercise.

Treatment:

Other: Exercise

High intensity exercise

Experimental group

Description:

Subjects in this group will participate in an exercise session that is representative of a training event experienced by cadets preparing for military competitions. All exercises will consist of a mix of running activities (≤ 400 m); body weight exercises, such as burpees and lunges; and exercises, such as crunches and squats utilizing 20.4 kg weight plates; with rapidly occurring exercise transitions to prevent recovery. The exercise bout will last a total of 100 min. Scheduled 5 min rests will be inserted at 30, 60, and 85 min. During the scheduled rest periods, subjects will be encouraged to consume water and/or the carbohydrate/electrolyte drink. The researchers will obtain a blood sample from you prior to and immediately following the exercise, as well as one, four and six hours post-exercise.

Treatment:

Other: Exercise