Tachykinin and Kisspeptin Expression in Human Granulosa and Cumulus Cells

IVI Sevilla

Status: Completed

Conditions: Infertility

Treatments: Procedure: Ovarian puncture

Study type: Interventional

Funder types: Other

Identifiers

NCT02877992

IVISEVILLA

Details and patient eligibility

About

The purpose of this project is to study the presence of expression differences - at RNA and protein level - of different members of the tachykinin family and kisspeptin and their receptors, between fertile women and infertile patients with different etiologies.

Enrollment: 236 patients

Sex: Female

Ages: 18 to 45 years old

Volunteers: Accepts Healthy Volunteers

Inclusion and exclusion criteria

Two groups of subjects (each one with different criteria):

1. Oocyte donors

   Inclusion Criteria:

   • Age between 18 and 34 years
   • Normal caryotype
   • Normal psychological test

   Exclusion Criteria:

   • Presence of hereditary diseases
   • Beta-thalassemia
   • Cystic fibrosis
   • Human immunodeficiency virus (HIV), Hepatitis B virus (HBV), Hepatitis C virus (HCV)

2. Infertility patients

Inclusion Criteria:

• Age between 18 and 45 years
• One of the following etiologies:
• Patient with PCOS according to Rotterdam Criteria or
• Patients with endometriosis stage I-IV or
• Patients with low ovarian response according to Bologna criteria or
• Patients with advances maternal age (between 38 and 45 years)

Trial design

Primary purpose: Basic Science

Allocation: Non-Randomized

Interventional model: Single Group Assignment

Masking: None (Open label)

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236 participants in 5 patient groups

Donors

Active Comparator group

Description:

Oocyte donors without infertility problems

Treatment:

Procedure: Ovarian puncture

PCOS

Experimental group

Description:

Patients with Polycystic Ovary Syndrome (PCOS) according to Rotterdam criteria

Treatment:

Procedure: Ovarian puncture

Endometriosis

Experimental group

Description:

Patients with endometriosis (I-IV)

Treatment:

Procedure: Ovarian puncture

Age

Experimental group

Description:

Patients with advanced maternal age (38 years or more)

Treatment:

Procedure: Ovarian puncture

Low ovarian response

Experimental group

Description:

Patients with low ovarian response according to Bologna criteria

Treatment:

Procedure: Ovarian puncture