Technical Optimization of Detection of KRAS, BRAF and NRAS Mutations on Tumor DNA Circulating in Metastatic Colorectal Cancer (CircuLOR-1)

Institut de Cancérologie de Lorraine

Status: Completed

Conditions: Metastatic Colorectal Cancer

Treatments: Other: KRAS, NRAS et BRAF mutation analysis from circulating plasma DNA

Study type: Interventional

Funder types: Other

Identifiers

NCT02827565

2015-A00922-47

Details and patient eligibility

About

The objective of this study is to optimize the search by next-generation sequencing (NGS) mutations in the KRAS, BRAF and NRAS on circulating tumor DNA and compare the genetic profiles obtained with those from tumors embedded in paraffin

Enrollment: 30 patients

Sex: All

Ages: 18+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Patients with metastatic or locally advanced colorectal carcinoma with a KRAS, BRAF or NRAS mutation
• Age ≥ 18 years
• Patient information and written informed consent form signed
• Patient must be affiliated to a social security system

Exclusion criteria

• Patients whose health-cons indicates a blood sample 10mL
• Age < 18 years
• Pregnant or breast feeding females
• Persons deprived of liberty or under supervision

Trial design

Primary purpose: Other

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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30 participants in 1 patient group

CircuLOR-1

Experimental group

Description:

KRAS (exons 2, 3 et 4), NRAS (exons 2, 3 et 4) and BRAF (exon 15) mutations

Treatment:

Other: KRAS, NRAS et BRAF mutation analysis from circulating plasma DNA