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Testicular Injection of Autologous Stem Cells for Treatment of Patients With Azoospermia

H

Hesham Saeed Elshaer

Status and phase

Unknown
Phase 2
Phase 1

Conditions

Azoospermia

Treatments

Biological: Stem Cells

Study type

Interventional

Funder types

Other

Identifiers

NCT02041910
RFC0550330 (Other Grant/Funding Number)
NCT0550330

Details and patient eligibility

About

Azoospermia is defined as the complete lack of sperm in the ejaculate. In humans, Azoospermia affects about 1% of the male population and may be seen in up to 20% of male infertility situations. In testicular Azoospermia the testes are abnormal, atrophic, or absent, and sperm production severely disturbed to absent. FSH levels tend to be elevated (hypergonadotropic) as the feedback loop is interrupted. The condition is seen in 49-93% of men with Azoospermia. The purpose of this study is to assess the ability of bone marrow derived stem cells to differentiate into germ cells and their role in treatment of testicular Azoospermia

Full description

This study is an open-label investigation of the efficacy of injection autologous adult bone marrow derived stem cells into the somniferous tubules or interstitial spaces of male testis with Azospermia. Sixty men with Azoospermia will be recruited in this study after a written informed consent.

The diagnosis of Azoospermia will be established on the basis of two semen analysis evaluations done at separate occasions; this will be followed by detailed history taking, physical examination and investigations. History taking will include general health, sexual health, past fertility, libido, sexual activity and previous exposure to surgery, drugs, mumps infection and irradiation. Physical examination includes genital and local examination for detection of signs of Klinefelter syndrome, testicular atrophy, absence of vas. Investigations include serum FSH, LH, karyotyping and testosterone levels, and may include testicular biopsy or transrectal ultrasound if indicated (Low levels of LH and FSH with low or normal testosterone levels are indicative of pretesticular problems, while high levels of gonadotropins indicate testicular problems. However, often this distinction is not clear and the differentiation between obstructive versus non-obstructive Azoospermia may require a testicular biopsy).

Subjects will be enrolled based on specific inclusion/exclusion criteria and evaluated at regular post transplant intervals. Human adult bone marrow derived stem cells will be transplanted by an andrological surgeon through a standard surgical approach. Subjects will be monitored frequently for a total of one year after adult bone marrow stem cells cell injection. For safety of participants, Bone Marrow Stem cells will be injected in one testis only and the other testis will be spared.

Enrollment

60 estimated patients

Sex

Male

Ages

25 to 60 years old

Volunteers

No Healthy Volunteers

Inclusion criteria

  • Patients with non-obstructive Azoospermia
  • Patients between 25 - 60 years old.

Exclusion criteria

  • Patients with obstructive Azoospermia
  • Men with previous surgery in testis
  • Men with infectious genital diseases and anatomical abnormalities of the genital tract
  • Those with major medical problems such as malignancy, hepatitis, etc.
  • Chromosomal aberration (e.g. Y microdeleion, trisomy....)

Trial design

Primary purpose

Treatment

Allocation

Randomized

Interventional model

Single Group Assignment

Masking

None (Open label)

60 participants in 2 patient groups

Stem Cells
Experimental group
Description:
60 ml of Bone marrow will aspirated for stem cells isolation and preparation. 5 ml of stem cells prepared according to GMP rules injected into testis.
Treatment:
Biological: Stem Cells
Biological: Stem Cells
Stem Cells Injection
Experimental group
Description:
Stem Cell Dose 3-5 Million Autologous MSCs Injected into testis.
Treatment:
Biological: Stem Cells
Biological: Stem Cells

Trial contacts and locations

1

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Central trial contact

Hesham El Shaer, MD

Data sourced from clinicaltrials.gov

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