The Association Between Calretinin and the Function of Ganglion Cells in Hirschsprung Disease

St. Justine's Hospital

Status: Unknown

Conditions: Hirschsprung Disease; Hypoganglionosis; Ganglion; Hirschsprung Disease, Long-Segment

Treatments: Diagnostic Test: Calretinin staining

Study type: Observational

Funder types: Other

Identifiers

NCT04149093

2020-2597

Details and patient eligibility

About

This study aims to compare the outcomes of patients with long segment Hirschsprung disease or total colonic aganglionosis who had negative calretinin staining and positive ganglion cells on the proximal resection margins to those who had both positive findings.

Enrollment: 50 estimated patients

Sex: All

Ages: Under 18 months old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Confirmed histopathological diagnosis of Hirschsprung disease from January 1, 1990 to August 31, 2019
• Long-segment Hirschsprung disease (type A and B)
• Total colonic aganglionosis (TCA)
• Surgery at CHUSJ
• Tissue blocks of proximal resection margin available for pathological analysis
• Minimum 1 month of postoperative follow-up

Exclusion criteria

• Short-segment Hirschsprung disease (conventional form, rectosigmoid junction)
• Total colonic with small bowel aganglionosis (TCSA)
• Surgery done at another institution
• Early deaths
• Lost to follow-up
• No documented post-operative outcome