The Characteristics and Treatment Mechanism of Vascular Grafts in Elderly Patients With CAOD Through Single-cell Transcriptome Analysis

Yonsei University

Status: Enrolling

Conditions: Coronary Artery Occlusive Disease (CAOD)

Study type: Observational

Funder types: Other

Identifiers

NCT07053254

4-2022-0197

Details and patient eligibility

About

This study aims to establish a cardiovascular biobank using vascular grafts obtained from participants with coronary artery occlusive disease (CAOD). Through genomic and transcriptomic analyses, the study seeks to characterize the biological features of the vascular grafts and identify potential therapeutic targets and mechanisms related to vascular remodeling and stiffness.

Full description

This observational study will utilize discarded vascular graft tissues obtained during coronary artery bypass grafting (CABG) procedures in participants with coronary artery occlusive disease (CAOD). Our surgical team performs over 200 CABG operations annually, allowing for the consistent and ethical acquisition of excess graft tissue without requiring any additional procedures or interventions for participants.

Collected tissues will be processed as follows:

A portion of each graft will be preserved in RNAlater solution for molecular analyses, including total RNA sequencing, quantitative PCR, and western blotting.

Another portion will be fixed in formalin and embedded for histological examination, including immunohistochemistry (IHC), to validate mechanisms of vascular remodeling and stiffness at the protein level.

This approach ensures that all biological samples are collected with minimal risk and no additional burden to participants, while enabling comprehensive multi-omic analysis to investigate the pathophysiological characteristics of vascular grafts in elderly CAOD patients.

Enrollment: 100 estimated patients

Sex: All

Ages: 19 to 100 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Adults aged 19 years or older, Patients scheduled to undergo coronary artery bypass grafting (CABG), Patients who voluntarily provide written informed consent for study participation, Patients who are able and willing to comply with study procedures during the study period

Exclusion criteria

• Pediatric or adolescent patients under 18 years of age, Patients requiring concomitant surgical correction for valvular heart disease, Patients undergoing reoperative (redo) cardiac surgery, Patients who decline to provide informed consent prior to surgery