The Characteristics of Oral Microbiota in Chronic Pancreatitis and Autoimmune Pancreatitis

Shanghai Jiao Tong University

Status: Enrolling

Conditions: Oral Microbiota; Pancreatitis; Pancreatic Cancer

Treatments: Other: 16S rRNA sequencing

Study type: Observational

Funder types: Other

Identifiers

NCT07015580

RuijinH202538

Details and patient eligibility

About

There are more than 700 different bacteria colonized in human oral cavity, which are collectively referred to as oral microbiota. Emerging evidence suggests that oral microbiota plays a series of important roles in human health, such as immune response, carcinogen metabolism and nutrient digestion. The changes of oral microbiota composition are closely related to the occurrence and development of pancreatic diseases. Previous studies have found that there are dense bacterial biofilms in the pancreatic duct of patients with calcified pancreatitis, including oral bacterial types. However, most studies only focused on the changes of gut microbiota in patients with chronic pancreatitis, and there were lack of research and description on the changes of oral microbiota in patients with chronic pancreatitis. In this study, we will extract and sequence bacterie's full-length 16S rRNA to describe the characteristics of oral microbiota in patients with chronic pancreatitis, confirming that there are changes in oral microbiota in patients with chronic pancreatitis, and compare the differences of oral microbiota in patients with chronic pancreatitis, pancreatic cancer and autoimmune pancreatitis.

Enrollment: 360 estimated patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• A dated and signed informed consent form cooperate to complete sample collection

Exclusion criteria

• Previous digestive system surgery; During pregnancy or lactation; Complicated with infectious diseases, malignant tumors or other digestive system diseases (such as inflammatory bowel disease, irritable bowel syndrome, abdominal diseases, gastroesophageal reflux, etc.); Antibiotics and immunosuppressive drugs were used within 30 days before sampling; Those who take probiotics daily.

Trial design

360 participants in 4 patient groups

chronic pancreatitis

Description:

Clinical data including medical history, clinical symptoms, imaging examination, laboratory examination will be collected. 3-5ml saliva sample of every patients will be collected for 16S rRNA sequencing of oral microbiota.

Treatment:

Other: 16S rRNA sequencing

autoimmune pancreatitis

Description:

Clinical data including medical history, clinical symptoms, imaging examination, laboratory examination will be collected. 3-5ml saliva sample of every patients will be collected for 16S rRNA sequencing of oral microbiota.

Treatment:

Other: 16S rRNA sequencing

pancreatic cancer

Description:

Gender, age (± 5 years), smoking and drinking are matched with patients with chronic pancreatitis. Clinical data including medical history, clinical symptoms, imaging examination, laboratory examination will be collected. 3-5ml saliva sample of every patients will be collected for 16S rRNA sequencing of oral microbiota. considering sugery or endoscopy may change the oral microbiota, the saliva sample will be collected in pantients with suspected pancreatic cancer. Only pantients with pathologically confirmed pancreatic cancer will be inclueded in final analysis

Treatment:

Other: 16S rRNA sequencing

healthy control

Description:

Gender, age (± 5 years), smoking and drinking are matched with patients with chronic pancreatitis. 3-5ml saliva sample of every participant will be collected for 16S rRNA sequencing of oral microbiota.

Treatment:

Other: 16S rRNA sequencing