The Construction of a Predictive Model for Gastric Cancer Immunotherapy Response Based on Tumor-Infiltrating Lymphocytes (TILs) and Histone H3K4me3 in the Tumor Microenvironment

Changzhi People's Hospital Affiliated to Changzhi Medical College

Status: Enrolling

Conditions: Gastric Cancer; Immunotherapy

Treatments: Diagnostic Test: Immunohistochemistry; Diagnostic Test: In situ hybridization with chromogenic detection; Diagnostic Test: Co-Immunoprecipitation (Co-IP) Technology; Diagnostic Test: Multiplex Immunofluorescence Technology

Study type: Observational

Funder types: Other

Identifiers

NCT07069842

2025K037

Details and patient eligibility

About

Gastric cancer is one of the cancers with a high mortality rate globally, and its treatment outcomes urgently need to be improved. The emergence of immunotherapy has broken through the bottleneck of chemotherapy for gastric cancer. However, the therapeutic efficacy of immunotherapy varies significantly among patients and still needs to be enhanced. Extensive research has demonstrated that the efficacy of immunotherapy for gastric cancer is significantly influenced by the tumor microenvironment (TME) and epigenetic modifications. The dynamic changes in tumor-infiltrating lymphocytes (TILs) and histone H3K4me3 may reshape the TME, thereby affecting the efficacy of immunotherapy. Based on these findings, this study proposes the scientific hypothesis that the density and spatial distribution of TILs in the TME, as well as the level of H3K4me3 modification, may serve as key biomarkers for predicting the response of gastric cancer patients to immunotherapy. This project aims to delve into the potential mechanisms by which TILs and H3K4me3 enhance the efficacy of immune checkpoint inhibitors (ICIs) and to construct a predictive model for the response to immunotherapy in gastric cancer based on TILs and H3K4me3. The establishment of this model will help identify the patient population most likely to benefit from immunotherapy, facilitate personalized treatment, provide new ideas and approaches for the treatment of gastric cancer, and advance the field of gastric cancer immunotherapy.

Enrollment: 170 estimated patients

Sex: All

Ages: 18 to 75 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• 1.Diagnosed with histologically confirmed locally advanced (LA) unresectable or metastatic gastric/gastroesophageal junction (G/GEJ) adenocarcinoma, aged 18 to 75 years.

  2.Scheduled to receive first-line therapy with ICIs plus chemotherapy with or without anti-her-2 therapy.

  3.Have at least one measurable lesion as the target lesion per iRECIST V.1.1 criteria and an Eastern Cooperative Oncology Group (ECOG) performance status of 0-2

Exclusion criteria

• 1.History of secondary malignant tumors within 3 years prior to study initiation, or other types of brain/meningeal metastatic tumors.

2.Presence of concomitant diseases that, in the investigator's judgment, may seriously jeopardize their safety or interfere with study completion

Trial design

170 participants in 2 patient groups

High-Response Group

Treatment:

Diagnostic Test: Multiplex Immunofluorescence Technology

Diagnostic Test: Co-Immunoprecipitation (Co-IP) Technology

Diagnostic Test: In situ hybridization with chromogenic detection

Diagnostic Test: Immunohistochemistry

Non-Response Group

Treatment:

Diagnostic Test: Multiplex Immunofluorescence Technology

Diagnostic Test: Co-Immunoprecipitation (Co-IP) Technology

Diagnostic Test: In situ hybridization with chromogenic detection

Diagnostic Test: Immunohistochemistry