The Differences of Oocyte Maturation, Granulosa Cell Apoptosis Index Between Electroacupuncture And Sham Groups IVF

U

University of Indonesia (UI)

Status

Completed

Conditions

BCL2 Gene mRNA Overexpression
Oocyte Maturation
in Vitro Fertilization
Acupuncture

Treatments

Procedure: Electroacupuncture

Study type

Interventional

Funder types

Other

Identifiers

NCT03627533
Medical Acupuncture

Details and patient eligibility

About

Interventional studies compared electroacupuncture group versus sham electroacupuncture group to granulosa cell apoptosis index, oocyte maturation, GDF9 and BMP15 levels, and fertilization rate in in vitro fertilization program

Full description

The study was conducted at the Dr.Cipto Mangunkusumo National General Hospital, Indonesia in September - November 2018.The inclusion criteria are patients who underwent controlled ovarian stimulation with antagonist protocol, age 30-39 years, BMI <29kg / m2. Exclusion criteria were not having a skin infection at acupuncture point and not using a pacemaker. Then patients were randomly divided into two groups: electroacupuncture and sham electroacupuncture. Electroacupuncture therapy was done 6 times at: 2 times before and 4 times during controlled ovarian stimulation.Apoptotic index of granulosa cells were examined by quantitative real time PCR techniques for BAX/BCL-2 expression.Oocyte maturation and fertilization rate output parameters are examined by competent embryologists after ovum pick up while GDF9 and BMP15 levels were examined by quantitative real time PCR techniques for GDF9 and BMP15 mRNA expression

Enrollment

24 patients

Sex

Female

Ages

30 to 39 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

women underwent controlled ovarian stimulation with antagonist protocol BMI < 29 kg/m2 willing and sign the inform consent follow the research until completed

Exclusion criteria

having skin infection at acupoint location use a pacemaker

Trial design

Primary purpose

Treatment

Allocation

Randomized

Interventional model

Parallel Assignment

Masking

Triple Blind

24 participants in 2 patient groups

Electroacupuncture
Experimental group
Description:
Electroacupuncture therapy is done at the point of CV3 Zhongji, CV4 Guanyuan, and EXCA-1 Zigong with continuous wave, 2 Hz frequency for 30 minutes. Acupuncture manuals on MA-IC3 endocrine (ear points), GV20 Baihui, ST36 Zusanli, SP6 Sanyinjiao, BL57 Chengsan and KI3 Taixi for 30 minutes.
Treatment:
Procedure: Electroacupuncture
Sham electroacupuncture
Sham Comparator group
Description:
Sham electroacupuncture are done at same electroacupuncture point location but puncture are not done, also electro stimulator are turned off.
Treatment:
Procedure: Electroacupuncture

Trial contacts and locations

1

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Data sourced from clinicaltrials.gov

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