The Effect of Desfluran On Global Dna Methylation

Muğla Sıtkı Koçman University

Status: Completed

Conditions: DNA Damage

Treatments: Drug: Desflurane

Study type: Observational

Funder types: Other

Identifiers

NCT05111717

MSKU 02/VII

Details and patient eligibility

About

The purpose of this study is to demonstrate the molecular effect of desflurane on DNA by measuring the global DNA methylation level in patients under going lumbar spinal stenosis surgery under general anesthesia.

Full description

Forty patients scheduled for lumbar spinal surgery will include in the study. Peripheral venous blood samples will collect prior to the induction of anesthesia (T1) and after 120 minutes (T2). Anesthesia will maintaine with 40%-60% O2-air mixture and 6% desflurane. DNA will obtain from blood samples with the help of DNA isolation kit and global DNA methylation analysis will perform by ELISA method.

Enrollment: 40 patients

Sex: All

Ages: 18 to 65 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

1. Ability to understand and willingness to sign an informed consent form
2. Elective lumbar spinal surgery that will take at least 120 minutes,
3. American Society of Anesthesiologists (ASA) physical status I and II
4. Be between the ages of 18 and 65

Exclusion criteria

1. Patients with ASA physical status III or IV disease
2. Smoking or alcohol consumption
3. Chronic systemic disease (diabetes mellitus,chronic pulmonary disorders, chronic kidney and liver disorders
4. recently received radiation, chemotherapy
5. malignancy
6. blood transfusion in operation
7. use of antioxidant supplements
8. have received general anesthesia in the last three months
9. Patients with known occupational exposures (operating room personnel, chemical plant workers

Trial design

40 participants in 1 patient group

group 1

Description:

Peripheral venous blood samples will collected at two time points as follows: before the premedication and the induction of anesthesia (T₁): 120 minutes after the beginning of anesthesia (T₂). DNA will be obtained from blood samples , and global DNA methylation analysis will be done.

Treatment:

Drug: Desflurane