The Effects of Acute Total Sleep Deprivation Versus Normal Sleep on Metabolism

Uppsala University

Status: Completed

Conditions: Sleep Deprivation; Sleep

Treatments: Behavioral: Inhibitory task; Behavioral: Portion Size Task; Procedure: Oral glucose tolerance test; Procedure: Tissue samples; Procedure: Blood samples

Study type: Interventional

Funder types: Other

Identifiers

NCT01800253

SleepMetaJCCB2013

Details and patient eligibility

About

The study proposes to investigate whether acute total deprivation affects metabolism as measured through blood and peripheral tissues. Its aim is also to investigate how acute total sleep deprivation affects neurodegenerative markers, as well as hormones, memory performance and aspects of appetite regulation.

Full description

It is predicted that acute total sleep deprivation will affect gene expression and DNA methylation. It is also predicted that sleep deprivation will up-regulate ghrelin, and affect other neuroendocrine markers and hormones in a negative manner. It is further predicted that sleep deprivation will decrease participants' memory performance.

Enrollment: 17 patients

Sex: Male

Ages: 18 to 28 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Male
• Age 18-28y
• Healthy (self-reported) and not on medication
• Non-smoking
• Normal sleep-wake rhythm (i.e. 7-8 h per night, self-reported via diaries)

Exclusion criteria

• Major illness
• Taking any serious medications
• Any sleep conditions (e.g. irregular bedtimes, sleep complaints)
• Any dietary issues with the food items provided
• Current or history of endocrine, neurological or psychiatric disorders
• Shift work in the preceding three months or for a long duration
• Time travel over a significant number of time zones in the preceding two months
• Too much weight gain or weight loss in the preceding three months

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: None (Open label)

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17 participants in 2 patient groups

Total sleep deprivation

Experimental group

Description:

Participants will be required to stay up for the entire night before 'Blood Samples' and 'Tissue samples' will be taken and the 'Portion Size Task' and 'Inhibitory task' will be performed. This will then be followed by the 'Oral glucose tolerance test' with additional 'Blood Samples' to be taken as described for that test.

Treatment:

Behavioral: Inhibitory task

Procedure: Oral glucose tolerance test

Procedure: Tissue samples

Procedure: Blood samples

Behavioral: Portion Size Task

Sleep

Experimental group

Description:

Participants will have an 8-h sleep opportunity before 'Blood Samples' and 'Tissue samples' will be taken and 'Portion Size Task' and 'Inhibitory task' will be performed. This will be followed by the 'Oral glucose tolerance test' with additional 'Blood Samples' to be taken as described for that test.

Treatment:

Behavioral: Inhibitory task

Procedure: Oral glucose tolerance test

Procedure: Tissue samples

Procedure: Blood samples

Behavioral: Portion Size Task