The Effects of Antibiotic Combinations on Stem Cells

Eskisehir Osmangazi University

Status: Completed

Conditions: Stem Cells; Anti-Bacterial Agents

Treatments: Drug: Differentiation Markers

Study type: Observational

Funder types: Other

Identifiers

NCT04713436

2015-68

Details and patient eligibility

About

The purpose of this study was to evaluate the indirect effects of antibiotic combinations. SCAP viability, proliferation and apoptosis of the cells were evaluated. Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.

Full description

The purpose of this study was to evaluate the indirect effects of triple (TAP- ciprofloxacin, metronidazole and minocycline), double (DAP- ciprofloxacin and metronidazole) and modified triple antibiotic pastes (mTAP- ciprofloxacin, metronidazole and cefaclor) on mesenchmal stem cells derived from apical papilla (SCAP). SCAP were isolated from the impacted third molar teeth of the young adults. TAP, DAP and mTAP treatment (14-day medication and 28-day medication) were applied on SCAP and viability, proliferation and apoptosis of the cells were evaluated after four weeks. Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.

Enrollment: 3 patients

Sex: All

Ages: 16 to 22 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Patients with open apex third molar teeth for extraction indication

Exclusion criteria

• Patients with mature apex third molar teeth for extraction indication

Trial design

3 participants in 3 patient groups

Triple antibiotic paste(TAP)

Description:

ciprofloxacin, metronidazole and minocycline

Treatment:

Drug: Differentiation Markers

Doble antibiotic paste(DAP)

Description:

ciprofloxacin and metronidazole

Treatment:

Drug: Differentiation Markers

Modified triple antibiotic paste(mTAP)

Description:

ciprofloxacin, metronidazole and cefaclor

Treatment:

Drug: Differentiation Markers