The Effects of Nitrate, Caffeine, and Cold Exposure on Cardiovascular Function.

Loughborough University

Status: Completed

Conditions: Cold Exposure; Caffeine; Nitrate

Treatments: Dietary Supplement: Caffeine; Other: Cold pressor test; Dietary Supplement: TruBeet inorganic beetroot juice

Study type: Interventional

Funder types: Other

Identifiers

NCT06602921

LoughboroughUAM

Details and patient eligibility

About

Dietary inorganic nitrate supplementation may have positive implications on cardiovascular function. The aim of this project is to determine if nitrate supplementation can attenuate the effects that caffeine and cold exposure have on the cardiovascular system.

Full description

Acute caffeine consumption and cold exposure elicit negative effects on the cardiovascular system. Humans are regularly exposed to such conditions of increased cardiovascular strain, which may lead to elevated chronic cardiovascular risk. On the other hand, dietary inorganic nitrate supplementation is well established to improve cardiovascular function in healthy individuals. Thus, acute nitrate supplementation may compensate for the negative effects that caffeine and the cold place on the cardiovascular system. These effects will be measured using peripheral blood pressure, pulse wave analysis, heart rate variability, forearm blood flow, flow mediated dilation, and blood and saliva markers.

Enrollment: 24 patients

Sex: All

Ages: 18 to 40 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Blood pressure < 130 mmHg systolic and < 85 mmHg diastolic. BMI between 18.5 and 29.9 kg/m2. Females must be naturally menstruating (regular length cycle and menses) or using a form of combined hormonal contraception (for at least 6 months).

Exclusion criteria

• Existing cardiovascular or metabolic conditions. Experience any adverse reactions to experimental procedures or supplements. Allergies to supplements or breakfast items. Involved in any other studies. Blood pressure > 130 mmHg systolic and > 85 mmHg diastolic.

Trial design

Primary purpose: Prevention

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Double Blind

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24 participants in 4 patient groups, including a placebo group

Nitrate, caffeine, and cold pressor test (CPT)

Experimental group

Description:

All outcome variables will be measured for 24 participants in a trial where they receive 400 mg nitrate in the form of beetroot extract, 6 mg/kg caffeine powder, and undergo a 3 min cold pressor test.

Treatment:

Dietary Supplement: TruBeet inorganic beetroot juice

Other: Cold pressor test

Dietary Supplement: Caffeine

Nitrate, placebo caffeine, and CPT

Placebo Comparator group

Description:

All outcome variables will be measured for 24 participants in a trial where they receive 400 mg nitrate in the form of beetroot extract, 6 mg/kg cornflour (caffeine placebo), and undergo a 3 min cold pressor test.

Treatment:

Dietary Supplement: TruBeet inorganic beetroot juice

Other: Cold pressor test

Placebo nitrate, caffeine, and CPT

Placebo Comparator group

Description:

All outcome variables will be measured for 24 participants in a trial where they receive nitrate-depleted beetroot extract (placebo), 6 mg/kg caffeine powder, and undergo a 3 min cold pressor test.

Treatment:

Other: Cold pressor test

Dietary Supplement: Caffeine

Placebo nitrate, placebo caffeine, and CPT

Placebo Comparator group

Description:

All outcome variables will be measured for 24 participants in a trial where they receive nitrate-depleted beetroot extract (placebo), 6 mg/kg cornflour (caffeine placebo), and undergo a 3 min cold pressor test.

Treatment:

Other: Cold pressor test