The Impact of Leg Immobilization on Postabsorptive and Postprandial Muscle Protein Breakdown in Healthy Young Males

University of Exeter

Status: Completed

Conditions: Healthy

Treatments: Other: Postprandial; Other: Postabsorptive; Procedure: Immobilisation

Study type: Interventional

Funder types: Other

Identifiers

NCT03934632

180509/B/01

Details and patient eligibility

About

The present study will seek the quantify the simultaneous muscle protein synthesis and breakdown response with and without amino acid provision in humans following 2 days of immobilisation.

Enrollment: 24 patients

Sex: Male

Ages: 18 to 40 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Male
• 18-40 years of age
• Body mass index between 18.5 and 30

Exclusion criteria

• Any diagnosed metabolic impairment (e.g. type 1 or 2 Diabetes)
• Any diagnosed cardiovascular disease (e.g. deep vein thrombosis) or hypertension
• Chronic use of any prescribed or over the counter pharmaceuticals (that may modulate muscle protein metabolism)
• A personal or family history of epilepsy, seizures or schizophrenia
• Any known disorders in muscle metabolism
• Regular use of nutritional supplements
• Allergy to lidocaine
• Allergy to one or multiple amino acids
• Recent (within the last 6 months) or current musculoskeletal injury (e.g. leg fracture) as these could be exacerbated by the intervention e.g. unilateral leg immobilisation, or mean the participant is unable to use crutches.
• Having received or ingested a stable isotope tracer in the past

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Single Blind

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24 participants in 2 patient groups

Postabsorptive

Active Comparator group

Description:

Saline infusion to mimic postabsorptive circulating amino acid concentrations

Treatment:

Other: Postabsorptive

Procedure: Immobilisation

Postprandial

Active Comparator group

Description:

Amino acid infusion to mimic postprandial circulating amino acid concentrations

Treatment:

Other: Postprandial

Procedure: Immobilisation