The Influence of Different Concentrations of Glucose in Culture Media on Fertilization and Embryo Quality

Hillel Yaffe Medical Center

Status: Unknown

Conditions: Fertility

Treatments: Other: Universal IVF Medium + ISM1 Medium

Study type: Interventional

Funder types: Other

Identifiers

NCT00606073

HYMC IRB#31/2007

Details and patient eligibility

About

The aim of this study is to examine whether incubation of human oocytes until pronuclear stage, in different culture media containing high and low concentrations of glucose affect fertilization differently, not only in standard IVF cycles, but also after intracytoplasmatic sperm injection.In addition, we wish to determine whether exposure of oocytes to different concentrations of glucose until pronuclear stage has further effect on embryo quality and development until the blastocyst stage.

Enrollment: 100 estimated patients

Sex: All

Ages: 18 to 50 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Patients undergoing IVF treatment in our clinic during study period

Exclusion criteria

• Females older than 40 years
• Patients with less than 6 retrieved oocytes
• Male patients older than 50 years old
• Patients after testis biopsies

Trial design

100 participants in 4 patient groups

I

Active Comparator group

Description:

IVF Procedure-IVF Medium

Treatment:

Other: Universal IVF Medium + ISM1 Medium

II

Active Comparator group

Description:

IVF Procedure - ISM1 Medium

Treatment:

Other: Universal IVF Medium + ISM1 Medium

III

Active Comparator group

Description:

ICSI Procedure - IVF Medium

Treatment:

Other: Universal IVF Medium + ISM1 Medium

IV

Active Comparator group

Description:

ICSI Procedure - ISM1 Medium

Treatment:

Other: Universal IVF Medium + ISM1 Medium