The Influence of Different Concentrations of Glucose in Culture Media on In Vitro Fertilization (IVF) Outcome of Sibling Oocytes

Hillel Yaffe Medical Center

Status: Unknown

Conditions: Infertility

Treatments: Other: Sibling oocyte incubation

Study type: Observational

Funder types: Other

Identifiers

NCT02066584

0011-14HYMC

Details and patient eligibility

About

The importance of glucose in culture media on fertilization and embryo development is still controversial. Although it has been reported that glucose is essential for fertilization process and biosynthesis of membrane lipid and nucleic acids, comparatively high levels of glucose in culture media may cause developmental arrest of cleavage stage embryos. The aim of this retrospective study is to examine whether exposure of sibling oocytes to different concentrations of glucose in culture media has any further effect on IVF outcome.

Full description

This retrospective study will include data from either standard IVF or Intracytoplasmic Sperm Injection (ICSI) cycles, in which sibling oocytes were equally divided and incubated separately in one of the following pairs of culture media:

1. Universal IVF medium vs. ISM1 medium ( Origio, Medi-Cult)
2. P1 medium vs. ECM medium (Irvine).

Fertilization rate,as well as embryo cleavage rate and morphology will be evaluated.

Enrollment: 600 estimated patients

Sex: Female

Ages: 18 to 42 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Sibling oocytes from cycles where at least 4 oocytes were retrieved

Exclusion criteria

• Less than 4 oocytes retrieved
• Partner has undergone testicular sperm extraction (TESE)

Trial design

600 participants in 4 patient groups

Universal IVF Medium

Description:

An oocyte culture medium containing 5.55 Mm glucose (Origio, Medi-Cult)

Treatment:

Other: Sibling oocyte incubation

ISM1 Medium

Description:

An oocyte culture medium containing 1mM glucose ((Origio, MediCult)

Treatment:

Other: Sibling oocyte incubation

P1 Medium

Description:

An oocyte culture medium containing no glucose (Irvine)

Treatment:

Other: Sibling oocyte incubation

ECM Medium

Description:

An oocyte culture medium containing 0.5mM glucose (Irvine)

Treatment:

Other: Sibling oocyte incubation