The Influence of Sperm Morphology and Functional Indices on Blastocyst Development in Assisted Reproduction

Calla IVF Center

Status: Completed

Conditions: Aneuploidy; Blastocyst; in Vitro Fertilization; Spermatozoa, Abnormal

Treatments: Other: Sperm morphology indexes

Study type: Observational

Funder types: Other

Identifiers

NCT06801652

CALLA TERATOZOOSPERMIA

Details and patient eligibility

About

Samples from male partners of 120 infertile couples undergoing IVF were analyzed. Sperm morphology was assessed using the SpermBlue staining method, while TZI, MAI, and SDI were calculated. Embryo development was monitored until day 5 or 6. Statistical analyses included regression models and correlation coefficients to evaluate the predictive value of sperm indices on blastocyst development.

Full description

For spermatozoa selection we used ZyMot multi in all patients undergoing IVF. After intracytoplasmatic injection of spermatozoa we asses the blastocyst grading in day 5. NEQSI score meaning numerical embryonic quality score index was used for statistical purpose. The spermatozoa morphology indexes were: TZI (teratozoospermia index), MAI (multiple anomalies index), SDI (sperm deformity index) and analyzed with CASA analyzer. All these indexes were assessed in patients and correlated in all groups: patients that had a positive pregnancy test, patients that had a negative pregnancy rate, patients that had no blastocyst in day 5 and patients that had all aneuploid embyos.

Enrollment: 120 patients

Sex: All

Ages: 21 to 40 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• couples with an infertility diagnosis that underwent an IVF procedure and had obtained at least one blastocyst

Exclusion criteria

• donors
• uterine abnormalities

Trial design

120 participants in 4 patient groups

Positive HCG pregnancy test after embryotransfer

Description:

Patients with sperm morphology indexes analyzed and had at least one good quality blastocyst that had a Positive HCG pregnancy test after embryo-transfer.

Treatment:

Other: Sperm morphology indexes

Negative HCG pregnancy test after embryotransfer

Description:

Patients with sperm morphology indexes analyzed and had at least one good quality blastocyst that had a Negative HCG pregnancy test after embryo-transfer.

Treatment:

Other: Sperm morphology indexes

No embryo obtained after IVF

Description:

Patients with sperm morphology indexes analyzed and had no blastocyst in day 5 or 6 after ICSI-IVF.

Treatment:

Other: Sperm morphology indexes

All embryos obtained are aneuploid

Description:

Patients with sperm morphology indexes analyzed and had all blastocyst of day 5/6 tested and aneuployd after ICSI-IVF.

Treatment:

Other: Sperm morphology indexes