The Lung Microbiome in Sarcoidosis

University of Michigan

Status: Completed

Conditions: Sarcoidosis

Treatments: Behavioral: Questionnaires; Procedure: Bronchoscopy; Other: Blood draw

Study type: Observational

Funder types: Other

Identifiers

NCT02520323

HUM00095799

Details and patient eligibility

About

The objective of this study is to determine the presence of bacterial species, and the immunologic and molecular mechanisms of granuloma formation that might be involved in the pathogenesis of sarcoidosis. The investigators will collect peripheral blood and bronchoalveolar lavage fluid samples to isolate bacterial DNA and blood monocytes and lymphocytes. Specimens will be obtained from patients with sarcoidosis, with non-sarcoid lung disease, and healthy volunteers to directly compare the bacterial species present and the characteristics of cells thought to be involved in granuloma formation.

Enrollment: 21 patients

Sex: All

Ages: 30 to 60 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Clinical diagnosis of sarcoidosis (30 subjects) or
• Clinical diagnosis of non-sarcoid interstitial lung disease (10 subjects) or
• Age-matched controls (30 subjects)
• Ability to provide informed consent

Exclusion criteria

• Any disease condition (such as anemia, unstable cardiac disease, etc.) that in the opinion of the subject's primary physician would put the patient at risk of harm from having 60 cc of blood drawn or for undergoing bronchoscopy for this study.
• Patients who are undergoing bronchoscopy for presumed infection.
• Pregnant women, women who may be pregnant, and women who are breastfeeding.
• Patients who have undergone lung transplantation.
• Persons who are unable or unwilling to undergo bronchoscopy, including those unable to be accompanied by a designated driver to the procedure.
• Persons who have taken antibiotics in the 90 days prior to consent.
• Known HIV, HCV, alcohol, or other substance abuse history.

Trial design

21 participants in 2 patient groups

Blood only

Description:

20 sarcoidosis subjects and 20 healthy controls will complete lifestyle questionnaires and have blood drawn for microbiome analyses.

Treatment:

Other: Blood draw

Behavioral: Questionnaires

Blood and BAL

Description:

10 sarcoidosis subjects, 10 healthy controls, and 10 subjects with non-sarcoid interstitial lung disease will complete lifestyle questionnaires and undergo blood sampling for microbiome analyses as well as bronchoscopy for bronchoalveolar lavage microbiome analyses.

Treatment:

Other: Blood draw

Behavioral: Questionnaires

Procedure: Bronchoscopy