The Microbiome Variability and Antibiotic Resistance of Chronic Suppurative Otitis

Ziv Hospital

Status

Unknown

Conditions

Suppurative Otitis Media

Study type

Observational

Funder types

Other

Identifiers

NCT03456739

0019-18-ZIV

Details and patient eligibility

About

ear swabs will be collected from 120 patients with established CSOM at the ear-nose-throat (ENT) outpatient clinic at the Ziv medical center, and 120 swabs will be taken from children with Otitis Media with Effusion (OME) undergoing elective tympanostomy tube insertion, serving as a control group. Ear swabs will be frozen at -80C until analysis.DNA will be purified from ear swabs, and amplified by PCR so that a barcoded 16S rRNA library from each subject will be generated. Using correlation analysis, we will compare between microbial compositions in chronic otorrhea patients vs. control groups.An additional ear swab will be collected from patients and cultured to test antibiotic resistance.

Enrollment

240 estimated patients

Sex

All

Ages

1 to 9 years old

Volunteers

No Healthy Volunteers

Inclusion and exclusion criteria

Inclusion otitis media with effusion,parental informed consent, age between 1 - 9 years, no known immune deficient disorder -

Exclusion Criteria:no parental consent, known immune disorder, current acute otitis media.