The Molecular Pathogenesis Study of Cataract

Eye & ENT Hospital of Fudan University

Status: Enrolling

Conditions: Cataract; Pathogenesis

Study type: Observational

Funder types: Other

Identifiers

NCT05682001

CMP

Details and patient eligibility

About

To study the molecular pathogenesis of cataract and explore possible treatments，anterior lens capsules were collected from cataract patients

Full description

Anterior lens capsules were collected from the patients with DC and ARC, and transparent crystals of cadaveric eyes. M6A epitranscriptomic microarray was performed to investigate the altered m6A modifications and determine the differentially expressed mRNAs. The potential role of dysregulated m6A modification levels was predicted through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. Real-time polymerase chain reaction was performed to identify the dysregulated expression of RNA methyltransferases, demethylases, and readers.

Enrollment: 150 estimated patients

Sex: All

Ages: 18 to 90 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• diabetic cataract
• underwent cataract surgery

Exclusion criteria

• Combined with other eye diseases
• underwent other eye surgery

Trial design

150 participants in 3 patient groups

DC group

Description:

Anterior lens capsule tissues from DC patients

ARC group

Description:

Anterior lens capsule tissues from ARC patients

NC group

Description:

anterior lens capsules collected from three age-matched transparent crystals of cadaveric eyes