The Relationship of Adiponectin in Adipose Tissue, Thy-1 in Plaques, and Inflammatory Mediators With Cardiac Diseases

Far Eastern Memorial Hospital

Status: Completed

Conditions: Heart Surgery

Treatments: Procedure: VHD; Procedure: CAD

Study type: Interventional

Funder types: Other

Identifiers

NCT02519257

FEMH-96091

Details and patient eligibility

About

Atherosclerosis is an inflammatory process in which immune mechanisms interact with metabolic risk factors to initiate, propagate, and activate lesions in the arterial trees and is known as the main cause of coronary artery disease (CAD). Recently, there are more and more studies highlighted the potential importance of adipose tissue in relation to inflammation effects on CAD pathogenesis. However, it remains unclear whether Thy-1, adiponectin or any other inflammatory mediators in mediastinal adipose tissue contribute to CAD. In this study, we aim to analyze the expression of inflammatory mediators' expression via in vitro assay (3T3-L1 cell culture) and in vivo assay (human adipose tissues).

Full description

In addition, we'd like to investigate the association of the investigators' findings with clinical atherosclerotic risks, medications (statins or antiplatelet), and blood sugar. Now, we've established cell assay and successfully transformed mature adipocyte from 3T3-L1 cells. Besides, there were nighty-six patients enrolled into this study. Flow cytometry of Thy-1 and MCP-1 concentration in cultured medium by ELISA were also analyzed.

Enrollment: 96 patients

Sex: All

Volunteers: No Healthy Volunteers

Inclusion criteria

• coronary artery bypass grafting surgery
• valvular surgery

Exclusion criteria

• liver disease (GPT 2 times greater than normal limits)
• chronic renal insufficiency (Creatinine > 2.0 mg/dL)
• neoplastic diseases
• taking steroids
• congestive heart failure

Trial design

Primary purpose: Basic Science

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

---

96 participants in 2 patient groups

CAD

Experimental group

Description:

Patients with valve diseases proposed to have cardiac operations will be enrolled in this study, but those with congestive heart failure are excluded. Besides, those patients with valve diseases should have patent coronary arteries on coronary angiography.

Treatment:

Procedure: CAD

VHD

Experimental group

Description:

Patients with valve diseases proposed to have cardiac operations will be enrolled in this study, but those with congestive heart failure are excluded. Besides, those patients with valve diseases should have patent coronary arteries on coronary angiography.

Treatment:

Procedure: VHD