The Role of Congenital Mucosal Immune System in the Airways of Patients With Chronic Obstructive Pulmonary Disease

Medical University of Lodz

Status: Unknown

Conditions: Smoking, Tobacco; COPD Exacerbation

Treatments: Diagnostic Test: airway epithelial smear test

Study type: Observational

Funder types: Other

Identifiers

NCT04061122

RNN/206/16IKE

Details and patient eligibility

About

The purpose of the study is to explore the role of congenital mucosal immune system in the airways of patients with COPD including the impact of ILCs (Innate lymphoid cells) on metabolism of epithelial cells.During the research the following methods will be applied: measurement of Volatile Organic Compounds (VOCS), airway epithelial cytology and culture.

Full description

ILCs profiles, epithelium metabolism measured by VOCS and microbiome profiling (specific 16S rRNA-16S ribosomal RNA gene-targeted qPCR) will by determined in the airways of patients with COPD after the exacerbation. Microbiome profiling will be based on next generation sequencing (NGS) of the 16S rRNA encoding region.

Enrollment: 40 estimated patients

Sex: All

Volunteers: No Healthy Volunteers

Inclusion and exclusion criteria

Exclusion Criteria:

• Neoplasm
• Diabetes
• Astma
• acute airway infection in last 2 weeks

Trial design

40 participants in 4 patient groups

EX + / ECTS +

Description:

Patients suffering COPD exacerbation in last 7 days; active tobacco smokers

Treatment:

Diagnostic Test: airway epithelial smear test

EX - / ECTS +

Description:

Patients without COPD exacerbation in last 6 months; active tobacco smokers

Treatment:

Diagnostic Test: airway epithelial smear test

EX + / ECTS -

Description:

Patients suffering COPD exacerbation in last 7 days; quited tobacco smoking at least 12 months earlier

Treatment:

Diagnostic Test: airway epithelial smear test

EX - / ECTS -

Description:

Patients without COPD exacerbation in last 6 months; quited tobacco smoking at least 12 months earlier

Treatment:

Diagnostic Test: airway epithelial smear test