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The Role of SCUBE-1 in Ischemia-reperfusion Injury

D

Diskapi Yildirim Beyazit Training and Research Hospital

Status

Completed

Conditions

Diabetes
Gonarthrosis

Treatments

Diagnostic Test: spinal anesthesia

Study type

Observational

Funder types

Other

Identifiers

Details and patient eligibility

About

One consequence of tissue damage caused by tourniquet is ischemia-reperfusion injury. Short-term ischemia leads to vasodilatation and reactive hyperemia resulting in post-ischemic reperfusion microcirculation failure and tissue edema that extends from 30 minutes to 4 hours.

SCUBE-1 is a newly defined cell surface molecule. It emerges from many developing cells, including endothelium and platelets. Immunohistochemical demonstration of subendothelial matrix deposition in atherosclerosis in humans. We did not find any study that showed the post-ischemic regression of scube 1, which was shown to be significantly higher in ischemic events in the literature.

in this study is aimed to investigate the location / sensitivity of SCUBE-1 in diabetics and nondiabetics after application of regional anesthesia for ischemia-reperfusion injury induced by tourniquet application in knee prosthesis attempts in our aimed patients and compare this with other total antioxidant status (TAS) and MDA of ischemia-reperfusion parameters

Full description

After being taken to the operation room, the patient will be divided into two groups as group D (diabetic) and group K (control) by standardized anesthesia monitoring with 5-lead electrocardiogram (ECG), peripheral oxygen saturation (SpO2) and noninvasive blood pressure measurements.

In both groups, spinal anesthesia will be applied in a lateral decubitus position using a 22 gauge Quincke spinal needle from the interspinal space as 0.5% heavy marcaine 10-12.5 mg 1 min. Atropine 0.5 mg and ephedrine 5 mg should be administered if bradycardia develops in both groups. The disease tourniquet will be applied after induction, in accordance with the literature, to be above 150 mmHg of the systolic blood pressure.

Enrollment

15 patients

Sex

All

Ages

18 to 70 years old

Volunteers

No Healthy Volunteers

Inclusion criteria

  • Diabetes mellitus
  • knee replacement surgery
  • using a pneumatic tourniquet

Exclusion criteria

  • coronary artery disease
  • renal disorders
  • cognitive disorders
  • bleeding diathesis
  • who have passed general anesthesia
  • anti-inflammatory drugs treatment
  • abnormal HbA1c values

Trial design

15 participants in 2 patient groups

diabetic
Description:
the patients must have diabetic disease
Treatment:
Diagnostic Test: spinal anesthesia
control
Description:
the patients must not have diabetic
Treatment:
Diagnostic Test: spinal anesthesia

Trial contacts and locations

1

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Data sourced from clinicaltrials.gov

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