The Utility of Multiplex PCR for Detection of DNA in Neutropenic Patients With Hematological Malignancies (MedIso)

Vaud University Hospital Center

Status: Completed

Conditions: Neutropenia

Study type: Observational

Funder types: Other

Identifiers

NCT01669005

EK 324/06

Details and patient eligibility

About

Objectives: Invasive infections are the leading cause of morbidity and mortality in patients treated for hematological malignancies. Blood cultures are often negative in neutropenic patients because of low-burden of organisms, previous antibiotic therapy or non-infectious reason of fever. More rapid, accurate and sensitive diagnostic tools are needed.

Hypothesis: Multiplex real-time PCR may detect microbial DNA in neutropenic patients before febrile episode. To investigate this hypothesis, EDTA-blood was routinely collected for multiplex PCR at admission and 3x/week thereafter until discharge or recovery from neutropenia

Enrollment: 84 patients

Sex: All

Volunteers: No Healthy Volunteers

Inclusion criteria

• patients aged ≥18 years
• autologous or allogeneic hematopoietic stem cells transplantation or induction/consolidation chemotherapy performed

Exclusion criteria

• No blood samples were taken

Trial design

84 participants in 1 patient group

Bone marrow transplant unit patients

Description:

hospitalized patients in the bone marrow transplant unit for an autologous or allogeneic hematopoietic stem cells transplantation or induction/consolidation chemotherapy