Time-course of Mitochondrial Biogenic Gene and Protein Expression in Exercised Human Skeletal Muscle

Brendon Gurd, PhD

Status: Unknown

Conditions: Mitochondrial Biogenesis

Treatments: Other: HI; Other: LO

Study type: Interventional

Funder types: Other

Identifiers

NCT03975777

QMPL-TC-19

Details and patient eligibility

About

This study examines the impact of exercise intensity on the 12-hour time-course of mitochondrial biogenic gene and protein expression in human skeletal muscle. Briefly, participants will perform two experimental sessions involving an acute bout of work-matched low- or high-intensity interval cycling exercise in a randomized crossover fashion, two weeks apart. Skeletal muscle biopsies will be obtained from the vastus lateralis (3 from each leg) before and 1, 3, 6, 9, and 12 hours post-exercise. Changes in the expression of various transcription factors and mitochondrial proteins will be examined at the mRNA and protein level to determine the time-course of changes in these factors differs between exercise protocols of different intensities.

Enrollment: 10 estimated patients

Sex: Male

Ages: 18 to 30 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• recreationally active (no more than three hours of structured weekly physical activity)
• not currently involved in a systematic training program for improving cardiorespiratory fitness, muscular strength, and/or sport-specific skills
• body mass index < 30 kg/m2

Exclusion criteria

• presence of cardiometabolic disease
• taking regular prescription medication for the treatment of cardiometabolic disease
• current cigarette smoker

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Double Blind

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10 participants in 2 patient groups

low-intensity exercise - high-intensity exercise

Experimental group

Description:

low-intensity exercise session followed by a high-intensity exercise session separated by a minimum of 14 days

Treatment:

Other: LO

Other: HI

high-intensity exercise - low-intensity exercise

Experimental group

Description:

high-intensity exercise session followed by a low-intensity exercise session separated by a minimum of 14 days

Treatment:

Other: LO

Other: HI