Time Lag for Oocyte Denudation: A Better Choice for ICSI Outcome?!

Ahmed Saad

Status: Completed

Conditions: Infertility, Female

Treatments: Procedure: denudation time lag

Study type: Observational

Funder types: Other

Identifiers

NCT04185623

Hawaa-8

Details and patient eligibility

About

This study aiming to investigate if difference in the timing of cumulus removal post retrieval would have any critical impact on fertilization and embryonic development.

Full description

Patients under 37 year old who were treated with controlled ovarian stimulation for ICSI were randomly divided into 2 groups; Group A: Oocytes were denudated immediately after oocyte retrieval , Group B: Oocytes were denudated 2 hours after retrieval and both groups were microinjected at 4 hours from ovum pickup. Microinjection was performed on all matured oocytes only and they were cultured to blastocyst stage.The rates of maturation, fertilization, good quality blastulation, chemical pregnancy and clinical pregnancy were analysed and compared between the two groups.

Enrollment: 300 patients

Sex: Female

Ages: 24 to 37 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Women 24-37 years old
• With good ovarian reserve
• Induction using Long agonist protocol
• unexplained infertility

Exclusion criteria

• severe male factor
• Testicular samples for ICSI
• poor ovarian reserve
• no of oocytes collected less than 4

Trial design

300 participants in 2 patient groups

Group A (immediate denudation)

Description:

Oocytes were denudated immediately after oocyte retrieval and were microinjected at 4 hours from ovum pickup. Microinjection was performed on all matured oocytes only and they were cultured to blastocyst stage.The rates of maturation, fertilization, good quality blastulation, chemical pregnancy and clinical pregnancy were analysed and compared between the two groups.

Treatment:

Procedure: denudation time lag

Group B ( 2hs denudation after ovum pickup)

Description:

Oocytes were denudated 2 hours after retrieval and were microinjected at 4 hours from ovum pickup. Microinjection was performed on all matured oocytes only and they were cultured to blastocyst stage.The rates of maturation, fertilization, good quality blastulation, chemical pregnancy and clinical pregnancy were analysed and compared between the two groups.

Treatment:

Procedure: denudation time lag