Tissue Plasminogen Activator & Plasminogen Activator Inhibitor Type-1 In Gingival Tissues Of Chronic Periodontitis

Cairo University (CU)

Status: Completed

Conditions: Chronic Periodontitis

Treatments: Procedure: Open Flap Debridement

Study type: Observational

Funder types: Other

Identifiers

NCT03803176

12081976

Details and patient eligibility

About

Tissue Plasminogen Activator and Plasminogen Activator Inhibitor Type-1 Gene Expression will be evaluated clinically and histopathologically through Hematoxylin and eosin as well as by quantitative reverse transcription polymerase chain reaction in the gingival tissues of chronic periodontitis in an attempt to investigate whether the expression of these proteins could be involved in the disease pathogenesis or not, and to assess as well if their expression will be affected by the applied surgical periodontal treatment.

Full description

30 subjects were enrolled in this study and divided into: Group I (Control Group): 10 periodontally healthy volunteers, Group II (Chronic Periodontitis Group): 20 patients suffering severe CP and Group III (Treated Group): 20 patients who were scheduled for OFD. Gingival samples were collected from all subjects at baseline and at 3 months after OFD from the CP patients. Then, Hematoxylin and eosin (H&E) stained slides were examined and gene expression levels of t-PA and PAI-1 were measured in the gingiva through quantitative reverse transcription polymerase chain reaction (RT-PCR).

Enrollment: 30 patients

Sex: All

Ages: 30 to 50 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Patients with severe chronic periodontitis
• Pocket depth (PD) of ≥5 mm
• Clinical attachment level (CAL) ≥5 mm

Exclusion criteria

• Pregnant women
• Subjects taking any type of medication and/or antibiotic therapy during the 3 months before the study
• Subjects who received periodontal treatment within the past 6 months
• Smokers

Trial design

30 participants in 2 patient groups

Periodontally healthy subjects

Description:

Healthy subjects who attended the restorative dental clinic and have clinically healthy gingiva with zero plaque index (PI), gingival index (GI), and CAL (≤3 mm PD). Oral hygeine instructions will be given to these patients.

Chronic Periodontitis

Description:

Patients with severe Chronic Periodontitis having a pocket depth (PD) of ≥5 mm and a clinical attachment level (CAL) ≥5 mm. Open flap debridement (surgical periodontal therapy) will be carried out for these patients after scaling \& root planing.

Treatment:

Procedure: Open Flap Debridement